Transfusion Medicine and Hemotherapy

Review Article · Übersichtsarbeit

Free Access

The Stromal Activity of Mesenchymal Stromal Cells

Wagner W.a, b · Saffrich R.a · Ho A.D.a

Author affiliations

a Department of Medicine V, bDepartment of Physiology and Pathophysiology, University of Heidelberg, Germany

Corresponding Author

Dr. Dr. Wolfgang Wagner, Department of Medicine V University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany Tel. +49 6221 56-6614, Fax -5813, E-mail wolfgang_wagner@med.uni-heidelberg.de

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Transfus Med Hemother 2008;35:185–193

Abstract

The mechanism that regulates self-renewal and differentiation of hematopoietic stem cells (HSC) is a central question in stem cell biology that might ultimately lead to reliable protocols for in vitro expansion of HSC. Cellular fate is governed by cell-cell interaction with the microenvironment in the bone marrow, the stem cell niche. Mesenchymal stromal cells (MSC) are precursors of the cellular components, and they secrete extracellular matrix proteins of the bone marrow stroma. Therefore, MSC feeder layer might provide a suitable in vitro model system for the stem cell niche. In vitro assays demonstrate that MSC maintain the stem cell function of HSC and that MSC from bone marrow have a higher hematopoiesis supportive activity than MSC from adipose tissue. Cocultivation with MSC might pave the way for expansion of long-term repopulating HSC, and various clinical trials indicate that co-transplantation of HSC and MSC might enhance engraftment. Thus, MSC are promising tools to elucidate the underlying mechanism of the cellular microenvironment. The large variety of preparative protocols for isolation and cultivation of MSC affects their stromal activity. Standardized isolation methods and molecular characterization of MSC are of utmost importance for reproducible isolation of hematopoiesis supportive stromal cells and for their potential clinical application.

© 2008 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Review Article · Übersichtsarbeit

Received: October 02, 2007
Accepted: December 06, 2007
Published online: May 16, 2008
Issue release date: June 2008

Number of Print Pages: 9
Number of Figures: 2
Number of Tables: 0

ISSN: 1660-3796 (Print)
eISSN: 1660-3818 (Online)

For additional information: https://www.karger.com/TMH


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