Transfusion Medicine and Hemotherapy

Review Article · Übersichtsarbeit

Free Access

Phenotypic Characterization of Mesenchymal Stem Cells from Various Tissues

Rojewski M. · Weber B. · Schrezenmeier H.

Author affiliations

Universität Ulm, Institut für Transfusionsmedizin und Institut für Klinische Transfusionsmedizin und Immungenetik gemeinnützige GmbH, DRK Blutspendedienst Baden-Württemberg – Hessen, Ulm, Germany

Corresponding Author

Dr. Markus Rojewski, Institut für Transfusionsmedizin und Institut für Klinische Transfusionsmedizin und Immungenetik, gemeinnützige GmbH Universität Ulm, Helmholtzstraβe 10, 89081 Ulm, Germany, Tel. +49 731 150-6833, Fax -500, E-mail markus.rojewski@uni-ulm.de

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Transfus Med Hemother 2008;35:168–184

Abstract

Research on expanded human stem cells has become an increasing field of interest during the last decade. The increasing interest in adult stem cells, especially mesenchymal stem and mesenchymal stromal cells, in hematology and regenerative medicine is also based on the simplicity of isolation and ex vivo expansion of these cells. These processes require an adequate quality control of source and product. In this review, we summarize various different attempts to characterize mesenchymal stem cells based on surface protein expression by flow cytometry and to define multipotent subpopulations of mesenchymal stem cells for prospective isolation. The importance of defining functional assays and a unique marker panel to characterize mesenchymal stem cells for clinical trials as well as the factors that can modulate the marker expression is discussed.

© 2008 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Review Article · Übersichtsarbeit

Received: November 11, 2007
Accepted: April 23, 2008
Published online: May 16, 2008
Issue release date: June 2008

Number of Print Pages: 17
Number of Figures: 2
Number of Tables: 4

ISSN: 1660-3796 (Print)
eISSN: 1660-3818 (Online)

For additional information: https://www.karger.com/TMH


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