Cytogenetic and Genome Research
Autoradiographic study of RNA synthesis during meiotic prophase in the human oocyteHartung M. · Stahl A.Laboratoire d’Histologie et Embryologie II, Faculté de Médecine, Marseille
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Article / Publication Details
Accepted: July 27, 1977
Published online: May 02, 2008
Issue release date: 1978
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
Abstract
The incorporation of SH-uridine in oogonia and oocytes during meiotic prophase I was studied in three human fetuses 13, 18, and 19 weeks old. Following a 40- or 60-min pulse, intense nuclear and nucleolar labeling was observed in oogonia. During the preleptotene chromosome condensation stage, the heteropycnotic masses were unlabeled, while numerous silver grains were seen on the filaments persisting around these masses. During leptotene, chromosomal and nucleolar RNA synthesis was significant, but less than that in the oogonia. The rate of incorporation declined rapidly during zygotene and fell to a very low level at early pachytene. Throughout pachytene no nucleolar RNA synthesis was observed. Chromosomal RNA synthesis progressively recovered during middle pachytene, was of moderate intensity at late pachytene, and increased again at early diplotene. Nucleolar RNA synthesis was very intense at early diplotene, at the same time as nucleolar size and basophilia increased.
© 1978 S. Karger AG, Basel
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Article / Publication Details
Accepted: July 27, 1977
Published online: May 02, 2008
Issue release date: 1978
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
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