Cytogenetic and Genome Research

Original Article

Sequential silver staining and hybridization in situ on nucleolus organizing regions in human cells

Warburton D. · Henderson A.S.

Author affiliations

Department of Human Genetics and Development and Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, N.Y.

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Cytogenet Cell Genet 1979;24:168–175

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: January 03, 1979
Published online: May 06, 2008
Issue release date: 1979

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Chromosome preparations from eight individuals were first stained with silver nitrate to reveal the nucleolus organizing regions (NORs) and then hybridized in situ with ribosomal RNA. In six individuals the size of the silver-staining regions was positively correlated with the amount of label present after hybridization in situ. Thus the variation in silver-staining intensity among chromosomes was largely explained by variation in the number of rDNA gene copies per NOR. However, in two individuals this correlation was absent, suggesting that other factors can also influence the size of the silver-staining region.

© 1979 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: January 03, 1979
Published online: May 06, 2008
Issue release date: 1979

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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