Cytogenetic and Genome Research

Original Article

Construction of cloned libraries from RNA of human fetal tissues

Kurnit D.M.ab · Wentworth B.M.a · De Long L.c · Villa-Komaroff L.c

Author affiliations

aGenetics Division, Children’s Hospital Medical Center, Boston, bGenetics Division, Brigham and Women’s Hospital, Boston, and cDepartment of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, Mass.

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Cytogenet Cell Genet 1982;34:193–203

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Published online: May 07, 2008
Issue release date: 1982

Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.

© 1982 S. Karger AG, Basel




Article / Publication Details

First-Page Preview
Abstract of Original Article

Published online: May 07, 2008
Issue release date: 1982

Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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