Cytogenetic and Genome Research
Original Article
Construction of cloned libraries from RNA of human fetal tissuesKurnit D.M.ab · Wentworth B.M.a · De Long L.c · Villa-Komaroff L.caGenetics Division, Children’s Hospital Medical Center, Boston, bGenetics Division, Brigham and Women’s Hospital, Boston, and cDepartment of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, Mass.
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Article / Publication Details
Published online: May 07, 2008
Issue release date: 1982
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
Abstract
We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.
© 1982 S. Karger AG, Basel
Article / Publication Details
Published online: May 07, 2008
Issue release date: 1982
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
Copyright / Drug Dosage / Disclaimer
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