Fetal Diagnosis and Therapy

 

Cell-Free Fetal DNA in Maternal Plasma during Physiological Single Male Pregnancies: Methodology Issues and Kinetics

Horinek A.a · Korabecna M.f · Panczak A.b · Gallova Z.U.g · Nouzova K.e · Calda P.c · Hancarova M.d

Author affiliations

a3rd Medical Department, bInstitute of Biology and Medical Genetics, and cDepartment of Gynaecology and Obstetrics, 1st Faculty of Medicine and General Teaching Hospital, dFaculty of Natural Sciences, Charles University, eGyncentrum, Prague, fDepartment of Biology, and gClinic of Obstetrics and Gynecology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic

Keywords: Cell-free fetal DNAMaternal plasmaNoninvasive diagnosisReal-time PCR

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Fetal Diagn Ther 2008;24:15–21
https://doi.org/10.1159/000132400

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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 14, 2006
Accepted: February 09, 2007
Published online: May 27, 2008
Issue release date: July 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 2

ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)

For additional information: https://www.karger.com/FDT

Abstract

Objective: To analyze methodological influences and characterize the concentrations of cell-free fetal DNA (cffDNA) circulating in maternal plasma at different gestational ages in physiological pregnancies. Methods: We investigated 238 independent samples from single male-bearing pregnancies of different gestation age. In the other 50 pregnancies, the samples were collected three times during pregnancy (at all trimesters) to evaluate the kinetics of cffDNA. The manual and automated DNA extraction methods (Roche) were compared. cffDNA was amplified using real-time PCR method and Y-specific sequences SRY and DYS14. Total cell-free DNA circulating in maternal plasma was determined by the use of the GADPH sequence. Results: The elevation in the concentration of cffDNA during pregnancy with the highest value in the third trimester was observed independently on the DNA extraction method and on the Y-specific amplified sequence. The same is documented for the percentage of fetal DNA in total cell-free DNA in maternal plasma. It increases also in successive trimesters (8.3, 10.7 and 23.2%). Conclusions: We discuss methodological problems and describe statistical parameters of cffDNA concentrations in maternal plasma during pregnancy as the basic information for comparison with pregnancies having a pathological outcome.

© 2008 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 14, 2006
Accepted: February 09, 2007
Published online: May 27, 2008
Issue release date: July 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 2

ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)

For additional information: https://www.karger.com/FDT

Copyright / Drug Dosage / Disclaimer

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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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2008, Vol.24, No. 1

July 2008

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