Gene Mapping, Cloning, and Sequencing
Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1→q13.2Levitt R.C.a · Liu Z.a · Nouri N.a · Meyers D.A.b · Brandriff B.c · Mohrenweiser H.M.c
aDepartment of Anesthesiology and Critical Care Medicine; bDepartment of Medicine, Johns Hopkins Medical Institutions, Baltimore MD; cHuman Genome Center, Biology and Biotechnology Research Program, Lawrence Livermore Laboratories, Livermore CA (USA)
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Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1 →q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82 % of the time with 12 alleles (166–190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYRl-cen.
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