Cytogenetic and Genome Research

Original Article

A stable marker chromosome with a cryptic centromere: evidence for centromeric sequences associated with an inverted duplication

Sacchi N.a · Magnani I.a · Fuhrman-Conti A.M.a · Monard S.P.b · Darfler M.c

Author affiliations

aDepartment of Biology and Genetics, Medical School, University of Milan, Milan (Italy); bImperial Cancer Research Fund, London (UK); and cLife Technologies, Inc., Gaithersburg, MD (USA)

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Cytogenet Cell Genet 1996;73:123–129

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 11, 1995
Published online: May 20, 2008
Issue release date: 1996

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Centromere activation, an important mechanism in karyotype evolution, is occasionally observed in some human chromosome rearrangements. We report a possible occurrence of centromere activation in a marker chromosome containing an atypical centromere associated with an inverted duplication of the region 14q32→qter. The marker chromosome’s reduced centromere lacks both the α and β satellite sequences usually found at normal centromeres. In an attempt to identify the centromeric sequences, the marker chromosome was flow-sorted and amplified by a degenerate oligonucleotide primer polymerase chain reaction. Reverse chromosome painting experiments showed that the marker chromosome contains sequences that are unique to the distal region of chromosome 14, as well as a low copy number of (centromeric) sequences that are also highly represented in the centromeres of chromosomes 18 and 19. These data suggest the activation of a novel centromere in the 14q32→qter region, very likely consequent to the duplication of the region itself.

© 1996 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 11, 1995
Published online: May 20, 2008
Issue release date: 1996

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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