Origins of Renal Physiology (Dedicated to Carl Gottschalk)
Vitalism and Synthesis of Urea
Kinne-Saffran E. · Kinne R.K.H.
From Friedrich Wöhler to Hans A. Krebs
Max-Planck-Institut für molekulare Physiologie, Dortmund, Germany
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In 1828, Friedrich Wöhler, a German physician and chemist by training, published a paper that describes the formation of urea, known since 1773 to be a major component of mammalian urine, by combining cyanic acid and ammonium in vitro. In these experiments the synthesis of an organic compound from two inorganic molecules was achieved for the first time. These results weakened significantly the vitalistic hypothesis on the functioning of living cells, although Wöhler, at that time, was more interested in the chemical consequences of isomerism than in the philosophical implications of his finding. However, the chemical synthesis observed by Wöhler does not represent the reaction which is employed in the mammalian liver for urea synthesis. The mechanism of this process was elucidated by the German physician Hans A. Krebs and his medical student Kurt Henseleit in 1932 and was shown to include the ornithine cycle. This ‘urea cycle’ is only observed in living cells; this apparently vitalistic phenomenon is caused by the compartmentalization of the various enzymatic reactions in mitochondria and cytosol, respectively.
Wöhler F: Über künstliche Bildung des Harnstoffs. Ann Phys Chem 1828;12:253–256.
Salkowski E: Ueber den Vorgang der Harnstoffbildung im Thierkörper und den Einfluss der Ammoniaksalze auf denselben. Z Physiol Chem 1877;1:1–5.
Knierim W: Beiträge zur Kenntnis der Bildung des Harnstoffs im thierischen Organismus. Z Biol 1874;10:263–294.
Schultzen O, Nencki M: Die Vorstufen des Harnstoffs im thierischen Organismus. Z Biol 1872;8:124–138.
McCane RA: Degradation of protein nitrogen. Physiol Rev 1930;11:2–52.
Krebs HA, Henseleit K: Untersuchungen über die Harnstoffbildung im Tierkörper. Z Physiol Chem 1932; 210:33–66.
Holmes FL: Hans Krebs. Oxford, Oxford University Press, 1991.
Ackermann D: Über den biologischen Abbau des Arginins zu Citrullin. Z Physiol Chem 1931;203:66–69.
Wallach O: Briefwechsel zwischen J. Berzelius und F. Wöhler im Auftrage der Königlichen Gesellschaft der Wissenschaften zu Göttingen. Leipzig, 1901, vol 1, p 206.
Wallach O: Briefwechsel zwischen J. Berzelius und F. Wöhler im Auftrage der Königlichen Gesellschaft der Wissenschaften zu Göttingen. Leipzig, 1901, vol 1, p 208.
Dumas JB: Über die Zusammensetzung des Harnstoffs. Ann Physik Chemie 1830;19:487–493.
Bugge G: Friedrich Wöhler; in Das Buch der grossen Chemiker. Weinheim, Verlag Chemie, 1965, vol 1, p 31–52.
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