Skin Pharmacology and Physiology
Original Paper
Claudin 2 mRNA and Protein Are Present in Human Keratinocytes and May Be Regulated by All-trans-Retinoic AcidTelgenhoff D. · Ramsay S. · Hilz S. · Slusarewicz P. · Shroot B.DFB Branded Pharmaceuticals, Central Research Division, Fort Worth, Tex., USA
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Article / Publication Details
Received: April 13, 2007
Accepted: December 18, 2007
Published online: May 29, 2008
Issue release date: July 2008
Number of Print Pages: 7
Number of Figures: 8
Number of Tables: 1
ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)
For additional information: https://www.karger.com/SPP
Abstract
Tight junctions are composed of claudins, occludins, junctional adhesion molecules and plaque proteins. Claudin 2 protein forms a cation-selective channel which confers increased permeability in renal epithelial cells and in the intestine where its expression is restricted to the leaky epithelium. Immunohistochemical staining revealed claudin 2 staining in the granular layer of adult epidermis. Analysis of Western blots revealed bands corresponding to the molecular weight of claudin 2 in Madin-Darby canine kidney II cells, human kidney, adult skin and neonatal keratinocytes. Reverse transcriptase polymerase chain reaction on mRNA and cDNA sequence analysis found a 99% sequence homology between our cDNA and human claudin 2 (NIH BLAST sequence). Further, we show that all-trans-retinoic acid increases the expression of claudin 2 in keratinocytes in a dose-dependent manner. The discovery of claudin 2 transcript and protein in the skin could be of importance in epidermal differentiation, barrier function and pathological conditions.
© 2008 S. Karger AG, Basel
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References
- Furuse M, Sasaki H, Tsukita S: Manner of interaction of heterogeneous claudin species within and between tight junction strands. J Cell Biol 1999;147:891–903.
- Chen Y, Lu Q, Schneeberger EE, Goodenough DA: Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in Ras-transformed Madin-Darby canine kidney cells. Mol Biol Cell 2000;11:849–862.
- Turksen K, Troy TC: Barriers built on claudins. J Cell Sci 2004;117:2435–2447.
- Brandner JM, Kief S, Grund C, Rendl M, Houdek P, Kuhn C, Tschachler E, Franke WW, Moll I: Organization and formation of the tight junction system in human epidermis and cultured keratinocytes. Eur J Cell Biol 2002;81:253–263.
- Furuse M, Hata M, Furuse K, Yoshida Y, Haratake A, Sugitani Y, Noda T, Kubo A, Tsukita S: Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell Biol 2002;156:1099–1111.
- Schneeberger EE, Lynch RD: Structure, function, and regulation of cellular tight junctions. Am J Physiol 1992;262:L647–L661.
- Gumbiner BM: Breaking through the tight junction barrier. J Cell Biol 1993;123:1631–1633.
- Anderson JM, Van Itallie CM: Tight junctions: closing in on the seal. Curr Biol 1999;9:R922–R924.
- Amasheh S, Meiri N, Gitter AH, Schoneberg T, Mankertz J, Schulzke JD, Fromm M: Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells. J Cell Sci 2002;115:4969–4976.
- Tang VW, Goodenough DA: Paracellular ion channel at the tight junction. Biophys J 2003;84:1660–1673.
- Lipschutz JH, Li S, Arisco A, Balkovetz DF: Extracellular signal-regulated kinases 1/2 control claudin-2 expression in Madin-Darby canine kidney strain I and II cells. J Biol Chem 2005;280:3780–3788.
- Enck AH, Berger UV, Yu AS: Claudin-2 is selectively expressed in proximal nephron in mouse kidney. Am J Physiol Renal Physiol 2001;281:F966–F974.
- Furuse M, Furuse K, Sasaki H, Tsukita S: Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells. J Cell Biol 2001;153:263–272.
- Baltes S, Nau H, Lampen A: All-trans-retinoic acid enhances differentiation and influences permeability of intestinal Caco-2 cells under serum-free conditions. Dev Growth Differ 2004;46:503–514.
- Madison KC: Barrier function of the skin: ‘la raison d’être’ of the epidermis. J Invest Dermatol 2003;121:231–241.
- Lee SH, Jeong SK, Ahn SK: An update of the defensive barrier function of skin. Yonsei Med J 2006;47:293–306.
-
Telgenhoff D, Shroot B: Further evidence of multifunctionality in the keratinocyte: The endothelial protein c receptor. J Invest Dermatol 2005;125:xviii–xix.
- Elias P: Stratum corneum defensive functions: an integrated view. J Invest Dermatol 2005;125:183–200.
- Bikle DD, Ng D, Tu CL, Oda Y, Xie Z: Calcium- and vitamin D-regulated keratinocyte differentiation. Mol Cell Endocrinol 2001;177:161–171.
- Benoit S, Toksoy A, Ahlmann M, Schmidt M, Sunderkotter C, Foell D, Pasparakis M, Roth J, Goebeler M: Elevated serum levels of calcium-binding S100 proteins A8 and A9 reflect disease activity and abnormal differentiation of keratinocytes in psoriasis. Br J Dermatol 2006;155:62–66.
- Hwang SM, Ahn SK, Menon GK, Choi EH, Lee SH: Basis of occlusive therapy in psoriasis: correcting defects in permeability barrier and calcium gradient. Int J Dermatol 2001;40:223–231.
- Lee SH, Choi EH, Feingold KR, Jiang S, Ahn SK: Iontophoresis itself on hairless mouse skin induces the loss of the epidermal calcium gradient without skin barrier impairment. J Invest Dermatol 1998;111:39–43.
- Baron JM, Heise R, Blaner WS, Neis M, Joussen S, Dreuw A, Marquardt Y, Saurat JH, Merk HF, Bickers DR, Jugert FK: Retinoic acid and its 4-oxo metabolites are functionally active in human skin cells in vitro. J Invest Dermatol 2005;125:143–153.
- Njar VC, Gediya L, Purushottamachar P, Chopra P, Belosay A, Patel JB: Retinoids in clinical use. Med Chem 2006;2:431–438.
- Kang S: The mechanism of action of topical retinoids. Cutis 2005;75:10–13, discussion 13.
- Winterfield L, Cather J, Cather J, Menter A: Changing paradigms in dermatology: nuclear hormone receptors. Clin Dermatol 2003;21:447–454.
- Njar VC: Cytochrome p450 retinoic acid 4-hydroxylase inhibitors: potential agents for cancer therapy. Mini Rev Med Chem 2002;2:261–269.
-
Peltonen S, Riehokainen J, Pummi K, Peltonen J: Tight junction components occludin, zo-1, and claudins-1, -4, and -5 in active and healing psoriasis; in Lindblad WJ (ed): 36th Annual European Society for Dermatological Research Meeting. Paris, Blackwell Synergy, 2006, vol 126, pp S1–S116.
Article / Publication Details
Received: April 13, 2007
Accepted: December 18, 2007
Published online: May 29, 2008
Issue release date: July 2008
Number of Print Pages: 7
Number of Figures: 8
Number of Tables: 1
ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)
For additional information: https://www.karger.com/SPP
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