Pharmacology
Original Paper
Profile of Drug-Metabolizing Enzymes in Human Ileum and ColonPacifici G.M.a · Franchi M.a · Gervasi P.c · Longo V.c · di Simplicio P.a · Temellini A.a · Giuliani L.bDepartments of aGeneral Pathology and bSurgery, Medical School, University of Pisa; cInstitute of Mutagenesis and Differentiation, CNR, Pisa; dDepartment of Environmental Biology, University of Siena, Italy
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Article / Publication Details
Received: May 24, 1988
Accepted: October 04, 1988
Published online: June 05, 2008
Issue release date: 1989
Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
Abstract
Six patients (4 women and 2 men, age between 60 and 90 years), subjected to right hemicolectomy, were gut donors. The mucosa was isolated from the last portion of the ileum and the first portion of the colon. Tissue specimens were free from pathological changes. The activities of the enzymes of phase I (NADPH cytochrome c reductase, ethoxycoumarin O-deethylase, aminopyrine N-demethylase, microsomal epoxide hydrolase, cytosolic epoxide hydrolase, glutathione reductase and glutathione peroxidase) and the enzymes of phase II (glutathionetransferase, glucuronyltransferase, acetyltransferase, thioltransferase, sulphotransferase and glyoxalase) were measured in the microsomal or cytosolic fractions obtained from ileum and colon mucosa. The activity in the ileum was higher than in the colon for NADPH cytochrome c reductase (p < 0.05) and cytosolic epoxide hydrolase (p < 0.001) (phase I enzymes), and glutathionetransferase (p < 0.02), sulphotransferase (p < 0.05) and glyoxalase (p < 0.02) (phase II enzymes). The other enzymes had similar activities in two mucosa. The distribution pattern of drug metabolizing enzymes cannot be considered as a single pattern in human ileum and colon because of the observed enzyme-dependent differences.
© 1989 S. Karger AG, Basel
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Article / Publication Details
Received: May 24, 1988
Accepted: October 04, 1988
Published online: June 05, 2008
Issue release date: 1989
Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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