Pharmacology

Original Paper

Profile of Drug-Metabolizing Enzymes in the Cortex and Medulla of the Human Kidney

Pacifici G.M.a · Viani A.a · Franchi M.a · Gervasi P.G.b · Longo V.b · Di Simplicio P.c · Temellini A.a · Romiti P.a · Santerini S.a · Vannucci L.a · Mosca F.a

Author affiliations

Departments of a General Pathology and dSurgical Pathology, Medical School, University of Pisa; bInstitute of Mutagenesis and Differentiation, CNR, Pisa; cDepartment of Environmental Biology, University of Siena, Italy

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Pharmacology 1989;39:299–308

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 26, 1989
Accepted: July 22, 1989
Published online: June 05, 2008
Issue release date: 1989

Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: https://www.karger.com/PHA

Abstract

The cortex and medulla were isolated from kidneys whose donors (5 men and 1 woman, aged between 44 and 68 years) were undergoing nephrectomy to remove a tumor. Kidneys with normal architecture for at least two thirds of the organ were included in the study. Tissue specimens used in our experiments were free from pathological changes. The activities of the following enzymes of phase INADPH cytochrome c reductase, aminopyrine N-demethylase, ethoxycoumarin O-deethylase, ethoxyresorufin O-deethylase, microsomal and cytosolic epoxide hydrolases, glutathione reductase and glutathione peroxidase, and those of the following enzymes of phase II glutathione transferase, glucuronyl transferase, sulphotransferase, acetyltransferase, thiomethyltransferase, thiopurinemethyltransferase, thioltransferase and glyoxalase were measured. The activity in renal cortex was significantly higher than in medulla for NADPH cytochrome c reductase, cytosolic epoxide hydrolase, glutathione reductase and glutathione peroxidase (phase I enzymes), and glutathione transferase, acetyltransferase, thiomethyltransferase, thiopurinemethyltransferase, thioltransferase and glyoxalase (phase II enzymes). The other enzymes had similar activity in cortex and medulla. The distribution pattern of drug-metabolizing enzymes in the human kidney cannot be considered as a single pattern because of the observed enzyme-dependent differences between cortex and medulla.

© 1989 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 26, 1989
Accepted: July 22, 1989
Published online: June 05, 2008
Issue release date: 1989

Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: https://www.karger.com/PHA


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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