Pharmacology
Original Paper
Stimulatory Effect of Beta-Alanyl-L-Histidinato Zinc on Bone Formation in Tissue CultureYamaguchi M. · Miwa H.Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan
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Article / Publication Details
Received: September 03, 1990
Accepted: November 18, 1990
Published online: June 06, 2008
Issue release date: 1991
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
Abstract
The present investigation was undertaken to clarify the in vitro effect of β-alanyl-L-histidinato zinc (AHZ) on bone metabolism in tissue culture. Calvaria were removed from weanling rats (3-week-old male) and cultured for periods up to 96 h in Dulbecco’s modified Eagle medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained 10–8 to 10–4 mol/l AHZ. The bone cellular zinc content was significantly increased in cultures with concentrations of AHZ greater than 10–6 mol/l. With 10–5 mol/l zinc sulfate, the bone cellular zinc content was significantly elevated. Bone calcium content was significantly increased by the presence of 10–7 to 10–4 mol/l AHZ. This increase was blocked by the presence of 10–7 mol/l cycloheximide. Bone alkaline phosphatase activity was elevated in the presence of AHZ (10–7 to 10–4 mol/l), whereas it did not significantly alter acid phosphatase activity. Bone collagen and DNA contents were significantly increased by 10–7 to 10–5 mol/l AHZ, while they were not significantly elevated by zinc sulfate (10–7 and 10–6 mol/l). The AHZ (10–5 mol/l)-induced increase in bone alkaline phosphatase activity and DNA content were prevented by 10–4 mol/l dipicolinate, a chelator of zinc. Furthermore, the AHZ (10–5 mol/l)-induced increase in bone alkaline phosphatase activity, collagen and DNA contents were blocked by 10–7 mol/l cycloheximide. These findings indicate that AHZ had a direct stimulatory effect on bone mineralization in vitro, and that bone protein synthesis was a necessary component of this response. The AHZ effect was more intensive than that of zinc sulfate.
© 1991 S. Karger AG, Basel
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Article / Publication Details
Received: September 03, 1990
Accepted: November 18, 1990
Published online: June 06, 2008
Issue release date: 1991
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: https://www.karger.com/PHA
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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