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Original Research

Intracoronary Delivery of Bone Marrow Cells to the Acutely Infarcted Myocardium

Optimization of the Delivery Technique

Meluzín J.a · Vlašín M.b · Groch L.a · Mayer J.c · Křen L.d · Raušer P.b · Tichý B.c · Horňáček I.a · Sitar J.a · Palša S.c · Klabusay M.c · Kořístek Z.c · Doubek M.c · Pospíšilová Š.c · Lexmaulová L.b · Dušek L.e

Author affiliations

a1st Department of Internal Medicine/Cardioangiology, St. Anna Hospital, ICRC, Masaryk University, bDogs and Cats Clinic, University of Veterinary and Pharmaceutical Science, cDepartment of Internal Medicine/Hematooncology, FH Brno, Masaryk University, dDepartment of Pathology, Masaryk University, and eCenter of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic

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Cardiology 2009;112:98–106

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Article / Publication Details

First-Page Preview
Abstract of Original Research

Received: November 22, 2007
Accepted: February 28, 2008
Published online: June 27, 2008
Issue release date: December 2008

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 0008-6312 (Print)
eISSN: 1421-9751 (Online)

For additional information: https://www.karger.com/CRD

Abstract

Objectives: Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique – intracoronary cell infusion. Methods: Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy. Results: The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test). Conclusion: The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.

© 2008 S. Karger AG, Basel


References

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Article / Publication Details

First-Page Preview
Abstract of Original Research

Received: November 22, 2007
Accepted: February 28, 2008
Published online: June 27, 2008
Issue release date: December 2008

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 0008-6312 (Print)
eISSN: 1421-9751 (Online)

For additional information: https://www.karger.com/CRD


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