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Advances in Recovery of Novel Biocatalysts from Metagenomes

Steele H.L.a · Jaeger K.E.b · Daniel R.c · Streit W.R.a

Author affiliations

aBiozentrum Klein Flottbek, Abteilung für Mikrobiologie & Biotechnologie, Universität Hamburg, Hamburg; bInstitut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, Jülich; cAbteilung für Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, Göttingen, Germany

Corresponding Author

Prof. Dr. W.R. Streit

Universität Hamburg, Biozentrum Klein Flottbek

Ohnhorststrasse 18, DE–22609 Hamburg (Germany)

Tel: +49 40 4281 6463, Fax +49 40 4281 6459

E-Mail wolfgang.streit@uni-hamburg.de

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J Mol Microbiol Biotechnol 2009;16:25–37

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Abstract

Metagenomics has accelerated the process of discovery of novel biocatalysts by enabling scientists to tap directly into the entire diversity of enzymes held within natural microbial populations. Their characterization has revealed a great deal of valuable information about enzymatic activity in terms of factors which influence their stability and activity under a wide range of conditions. Many of the biocatalysts have particular properties making them suitable for biotechnological applications. A diverse array of strategies has been developed to optimize each step of the process of generating and screening metagenomic libraries for novel biocatalysts. This review covers the diversity of metagenome-derived enzymes characterized to date, and the strategies currently being developed to optimize discovery of novel metagenomic biocatalysts.

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Published online: October 29, 2008
Issue release date: October 2008

Number of Print Pages: 13
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ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

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