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Advances in Recovery of Novel Biocatalysts from Metagenomes

Steele H.L.a · Jaeger K.E.b · Daniel R.c · Streit W.R.a

Author affiliations

aBiozentrum Klein Flottbek, Abteilung für Mikrobiologie & Biotechnologie, Universität Hamburg, Hamburg; bInstitut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, Jülich; cAbteilung für Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, Göttingen, Germany

Corresponding Author

Prof. Dr. W.R. Streit

Universität Hamburg, Biozentrum Klein Flottbek

Ohnhorststrasse 18, DE–22609 Hamburg (Germany)

Tel: +49 40 4281 6463, Fax +49 40 4281 6459

E-Mail wolfgang.streit@uni-hamburg.de

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J Mol Microbiol Biotechnol 2009;16:25–37

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Metagenomics has accelerated the process of discovery of novel biocatalysts by enabling scientists to tap directly into the entire diversity of enzymes held within natural microbial populations. Their characterization has revealed a great deal of valuable information about enzymatic activity in terms of factors which influence their stability and activity under a wide range of conditions. Many of the biocatalysts have particular properties making them suitable for biotechnological applications. A diverse array of strategies has been developed to optimize each step of the process of generating and screening metagenomic libraries for novel biocatalysts. This review covers the diversity of metagenome-derived enzymes characterized to date, and the strategies currently being developed to optimize discovery of novel metagenomic biocatalysts.

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  1. Amann RI, Ludwig W, Schleifer KH: Phylogenetic identification and in-situ detection of individual microbial cells without cultivation. Microbiol Rev 1995;59:143–169.
  2. Bell PJL, Sunna A, Gibbs MD, Curach NC, Nevalainen H, Bergquist PL: Prospecting for novel lipase genes using PCR. Microbiology 2002;148:2283–2291.
  3. Brady D, Beeton A, Zeevaart J, Kgaje C, van Rantwijk F, Sheldon RA: Characterisation of nitrilase and nitrile hydratase biocatalytic systems. Appl Microbiol Biotechnol 2004;64:76–85.
  4. Brady SF, Chao CJ, Clardy J: Long chain N-acyltyrosine synthases from environmental DNA. Appl Environ Microbiol 2004;70:6865–6870.
  5. Brady SF, Clardy J: Palmitoylputrescine, an antibiotic isolated from the heterologous expression of DNA extracted from Bromeliad tank water. J Nat Prod 2004;67:1283–1286.
  6. Brady SF, Clardy J: Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA. Angew Chem Int Ed 2005a;44:7063–7065.
  7. Brady SF, Clardy J: N-Acyl derivatives of arginine and tryptophan isolated from environmental DNA expressed in Escherichia coli. Org Lett 2005b;7:3613–3616.
  8. Brennan Y, Callen WN, Christoffersen L, Dupree P, Goubet F, Healey S, Hernández M, Keller M, Li K, Palackal N, Sittenfeld A, Tamayo G, Wells S, Hazlewood GP, Mathur EJ, Short JM, Robertson DE, Steer BA: Unusual microbial xylanases from insect guts. Appl Environ Microbiol 2004;70:3609–3617.
  9. Bronnenmeier K, Kern A, Liebl W, Staudenbauer WL: Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials. Appl Environ Microbiol 1995;61:1399–1407.
  10. Cantarella M, Cantarella L, Gallifuoco A, Spera A: Use of a UF-membrane reactor for controlling selectively the nitrile hydratase-amidase system in Microbacterium imperiale CBS 498-74 resting cells. Case study: benzonitrile conversion. Enzyme Microb Technol 2006;38:126–134.
  11. Carstens M, Vivier MA, van Rensburg P, Pretorius IS: Overexpression, secretion and antifungal activity of the Saccharomyces cerevisiae chitinase. Ann Microbiol 2003;53:15– 28.
  12. Chatterjee R, Yuan L: Directed evolution of metabolic pathways. Trends Biotechnol 2006;24:28–38.
  13. Collins T, Gerday C, Feller G: Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 2005;29:3–23.
  14. Copp JN, Neilan BA: The phosphopantetheinyl transferase superfamily: phylogenetic analysis and functional implications in Cyanobacteria. Appl Environ Microbiol 2006;72:2298–2305.
  15. Cottrell MT, Moore JA, Kirchman DL: Chitinases from uncultured marine microorganisms. Appl Environ Microbiol 1999;65:2553–2557.
  16. Cottrell MT, Wood DN, Yu L, Kirchman DL: Selected chitinase genes in cultured and uncultured marine bacteria in the α- and γ-subclasses of the proteobacteria. Appl Environ Microbiol 2000;66:1195–1201.
  17. Cottrell MT, Yu L, Kirchman DL: Sequence and expression analyses of Cytophaga-like hydrolases in a Western arctic metagenomic library and the Sargasso Sea. Appl Environ Microbiol 2005;71:8506–8513.
  18. Courtois S, Cappellano CM, Ball M, Francou F-X, Normand P, Helynck G, Martinez A, Kolvek SJ, Hopke J, Osburne MS, August PR, Nalin R, Guérineau M, Jeannin P, Simonet P, Pernodet J-L: Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Appl Environ Microbiol 2003;69:49–55.
  19. Curtis TP, Sloan WT: Exploring microbial diversity – a vast below. Science 2005;309:1331–1333.
  20. Daniel R: The metagenomics of soil. Nat Rev Microbiol 2005;3:470–478.
  21. DeSantis G, Zhu Z, Greenberg WA, Wong K, Chaplin J, Hanson SR, Farwell B, Nicholson LW, Rand CL, Weiner DP, Robertson DE, Burk MJ: An enzyme library approach to biocatalysis: development of nitrilases for enantioselective production of carboxylic acid derivatives. J Am Chem Soc 2002;124:9024–9025.
  22. Diaz-Torres ML, McNab R, Spratt DA, Villedieu A, Hunt N, Wilson M, Mullany P: Novel tetracycline resistance determinant from the oral metagenome. Antimicrob Agents Chemother 2003;47:1430–1432.
  23. Elend C, Leggewie C, Babiak P, Carballeira JD, Steele HL, Reymond J-L, Jaeger K-E, Streit WR: Isolation and biochemical characterization of two novel metagenome derived esterases. Appl Environ Microbiol 2006;72:3637–3645.
  24. Elend C, Steele HL, Schmeisser C, Streit WR: Isolation and characterization of a metagenome-derived and cold-active lipase with high stereospecificity for (R)-ibuprofen esters. J Biotechnol 2007;130:370–377.
  25. Entcheva P, Liebl W, Johann A, Hartsch T, Streit WR: Direct cloning from enrichment cultures, a reliable strategy for isolation of complete operons and genes from microbial consortia. Appl Environ Microbiol 2001;67:89–99.
  26. Eschenfeldt WH, Stols L, Rosenbaum H, Khambatta ZS, Quaite-Randall E, Wu S, Kilgore DC, Trent JD, Donnelly MI: DNA from uncultured organisms as a source of 2,5-di-keto-D-gluconic acid reductases. Appl Environ Microbiol 2001;67:4206–4214.
  27. Ferrer M, Beloqui A, Golyshina OV, Plou FJ, Neef A, Chernikova TN, Fernandez-Arrojo L, Ghazi I, Ballesteros A, Elborough K, Timmis KN, Golyshin PN: Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome. Biotechnol J 2007;2:207–213.
  28. Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Martins dos Santos VAP, Yakimov MM, Timmis KN, Golyshin PN: Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin. Chem Biol 2005a;12:895–904.
  29. Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Reyes-Duarte D, Martins dos Santos VAP, Strompl C, Elborough K, Jarvis G, Neef A, Yakimov MM, Timmis KN, Golyshin PN: Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora. Environ Microbiol 2005b;7:1996–2010.
  30. Friedrich MW: Stable-isotope probing of DNA: insights into the function of uncultivated microorganisms from isotopically labelled metagenomes. Curr Opin Biotechnol 2006;17:59–66.
  31. Gabor EM, Alkema WBL, Janssen DB: Quantifying the accessibility of the metagenome by random expression cloning techniques. Environ Microbiol 2004a;6:879–886.
  32. Gabor EM, de Vries EJ, Janssen DB: Construction, characterization, and use of small-insert gene banks of DNA isolated from soil and enrichment cultures for the recovery of novel amidases. Environ Microbiol 2004b;6:948–958.
  33. Gabor EM, de Vries EJ, Janssen DB: A novel penicillin acylase from the environmental gene pool with improved synthetic properties. Enzyme Microb Technol 2005;36:182–190.
  34. Gans J, Wolinsky M, Dunbar J: Computational improvements reveal great bacterial diversity and high metal toxicity in soil. Science 2005;309:1387–1390.
  35. Gillespie, DE, Brady SF, Bettermann AD, Cianciotto NP, Liles MR, Rondon MR, Clardy J, Goodman RM, Handelsman J: Isolation of antibiotics turbomycin A and B from a metagenomic library of soil microbial DNA. Appl Environ Microbiol 2002;68:4301–4306.
  36. Ginolhac A, Jarrin C, Gillet B, Robe P, Pujic P, Tuphile K, Bertrand H, Vogel TM, Perrière G, Simonet P, Nalin R: Phylogenetic analysis of polyketide synthase I domains from soil metagenomic libraries allows selection of promising clones. Appl Environ Microbiol 2004;70:5522–5527.
  37. Grant S, Sorokin DY, Grant WD, Jones BE, Heaphy S: A phylogenetic analysis of Wadi el Natrun soda lake cellulase enrichment cultures and identification of cellulase genes from these cultures. Extremophiles 2004;8:421–429.
  38. Gupta R, Beg QK, Lorenz P: Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 2002;59:15–22.
  39. Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM: Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 1998;5:R245–249.
  40. Healy FG, Ray RM, Aldrich HC, Wilkie AC, Ingram LO, Shanmugam KT: Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on ligocellulose. Appl Microbiol Biotechnol 1995;43:667–674.
  41. Henne A, Daniel R, Schmitz RA, Gottschalk G: Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilisation of 4-hydroxybutyrate. Appl Environ Microbiol 1999;65:3901–3907.
  42. Henne A, Schmitz RA, Bömeke M, Gottschalk G, Daniel R: Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on E. coli. Appl Environ Microbiol 2000;66:3113–3116.
  43. Hoster F, Schmitz JE, Daniel R: Enrichment of chitinolytic microorganisms: isolation and characterisation of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Appl Microbiol Biotechnol 2005;66:434–442.
  44. Howard MB, Ekborg NA, Weiner RM, Hutcheson SW: Detection and characterisation of chitinases and other chitin-modifying enzymes. J Ind Microbiol Biotechnol 2003;30:627–635.
  45. Hugenholtz P, Goebel BM, Pace NR: Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 1998;180:4765–4774.
  46. Jaeger K-E, Eggert T: Lipases for biotechnology. Curr Opin Biotechnol 2002;13:390–397.
  47. Jaeger K-E, Eggert T, Eipper A, Reetz MT: Directed evolution and the creation of enantioselective biocatalysts. Appl Microbiol Biotechnol 2001;55:519–530.
  48. Jiang Z, Wang H, Ma Y, Wei D: Characterisation of two novel lipase genes isolated directly from environmental sample. Appl Microbiol Biotechnol 2006;70:327–332.
  49. Kim J-N, Seo M-J, Cho E-A, Lee S-J, Kim S-B, Cheigh C-I, Pyun Y-R: Screening and characterisation of an esterase from a metagenomic library. J Microbiol Biotechnol 2005;15:1067–1072.
  50. Kim Y-J, Choi G-S, Kim S-B, Yoon G-S, Kim Y-S, Ryu Y-W: Screening and characterisation of a novel esterase from a metagenomic library. Protein Expr Purif 2006;45:315–323.
  51. Knietsch A, Bowein S, Whited G, Gottschalk G, Daniel R: Identification and characterisation of coenzyme B12-dependent glycerol hydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures. Appl Environ Microbiol 2003a;69:3048–3060.
  52. Knietsch A, Waschkowitz T, Bowien S, Henne A, Daniel R: Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli. Appl Environ Microbiol 2003b;69:1408–1416.
  53. Knietsch A, Waschkowitz T, Bowien S, Henne A, Daniel R: Metagenomes of complex microbial consortia derived from different soils as sources for novel genes conferring formation of carbonyls from short-chain polyols on Escherichia coli. J Mol Microbiol Biotechnol 2003c;5:46–56.
  54. Konarzycka-Bessler M, Jaeger K-E: Select the best: novel biocatalysts for industrial applications. Trends Biotechnol 2006;24:248–250.
  55. Lämmle K, Zipper H, Breuer M, Hauer B, Buta C, Brunner H, Rupp S: Identification of novel enzymes with different hydrolytic activities by metagenome expression cloning. J Biotechnol 2007;127:575–592.
  56. Lee CC, Kibblewhite-Accinelli RE, Wagschal K, Robertson GH, Wong DWS: Cloning and characterisation of a cold-active xylanase enzyme from an environmental library. Extremophiles 2006a;10:295–300.
  57. Lee M-H, Lee C-H, Oh T-K, Song JK, Yoon J-H: Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases. Appl Environ Microbiol 2006b;72:7406–7409.
  58. Lee S-W, Won K, Lim HK, Kim J-C, Choi GJ, Cho KY: Screening for novel lipolytic enzymes from uncultured soil microorganisms. Appl Microbiol Biotechnol 2004;65:720–726.
  59. Leggewie C, Henning H, Schmeisser C, Streit WR, Jaeger K-E: A novel transposon for functional screening of DNA libraries. J Biotechnol 2006;123:281–287.
  60. Leresche JE, Meyer H-P: Chemocatalysis and biocatalysis (biotransformation): some thoughts of a chemist and of a biotechnologist. Org Process Res Dev 2006;10:572–580.
  61. Li Y, Wexler M, Richardson DJ, Bond PL, Johnston AWB: Screening a wide host-range, waste-water metagenomic library in tryptophan auxotrophs of Rhizobium leguminosarum and of Escherichia coli reveals different classes of cloned trp genes. Environ Microbiol 2005;7:1927–1936.
  62. Liebeton K, Eck J: Identification and expression in E. coli of novel nitrile hydratases from the metagenome. Eng Life Sci 2004;4:557–562.
  63. Liebl W, Ruile P, Bronnenmeier K, Riedel K, Lottspeich F, Greif I: Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterisation of the recombinant enzymes. Microbiology 1996;142:2533–2542.
  64. Lim HK, Chung EJ, Kim J-C, Choi GJ, Jang KS, Chung YR, Cho KY, Lee S-W: Characterisation of a forest soil metagenome clone that confers indirubin and indigo production on Escherichia coli. Appl Environ Microbiol 2005;71:7768–7777.
  65. Lorenz P, Eck J: Metagenomics and industrial applications. Nat Rev Microbiol 2005;3:510–516.
  66. Lueders T, Manefield M, Friedrich MW: Enhanced sensitivity of DNA- and RNA-based stable isotope probing by fractionation and quantitative analysis of isopycnic centrifugation gradients. Environ Microbiol 2004;6:73–78.
  67. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS: Microbial cellulose utilisation: fundamentals and biotechnology. Microbiol Mol Biol Rev 2002;66:506–577.
  68. MacNeil IA, Tiong CL, Minor C, August PR, Grossman TH, Loiacono KA, Lynch BA, Phillips T, Narula S, Sundaramoorthi R, Tyler A, Aldredge T, Long H, Gilman M, Holt D, Osburne MS: Expression and isolation of antimicrobial small molecules from soil DNA libraries. J Mol Microbiol Biotechnol 2001;3:301–308.
  69. Martinez A, Kolvek SJ, Yip CLT, Hopke J, Brown KA, MacNeil IA, Osburne MS: Genetically modified bacterial strains and novel bacterial artificial chromosome shuttle vectors for constructing environmental libraries and detecting heterologous natural products in multiple expression hosts. Appl Environ Microbiol 2004;70:2452–2463.
  70. Michel G, Nyval-Collen P, Barbeyron T, Czjzek M, Helbert W: Bioconversion of red seaweed galactans: a focus on bacterial agarases and carrageenases. Appl Microbiol Biotechnol 2006;71:23–33.
  71. Mohn WW, Garmendia J, Galvao TC, de Lorenzo V: Surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (γ-hexachlorocyclohexane) into lacZ-based phenotypes. Environ Microbiol 2006;8:546–555.
  72. Nakamura CE, Whited GM: Metabolic engineering for the microbial production of 1,3-propanediol. Curr Opin Biotechnol 2003;14:454–459.
  73. Ouverney CC, Fuhrman JA: Marine planktonic Archaea take up amino acids. Appl Environ Microbiol 2000;66:4829–4833.
  74. Pakchung AAH, Simpson PJL, Codd R: Life on Earth. Extremophiles continue to move the goal posts. Environ Chem 2006;3:77–93.
  75. Piel J, Butzke D, Fusetani N, Hui D, Platzer M, Wen G, Matsunaga S: Exploring the chemistry of uncultivated bacterial symbionts: antitumour polyketides of the pederin family. J Nat Prod 2005;68:472–479.
  76. Piel J, Hui D, Fusetani N, Matsunaga S: Targetting modular polyketide synthases with iteratively acting acyltransferases from metagenomes of uncultured bacterial consortia. Environ Microbiol 2004a;6:921–927.
  77. Piel J, Hui D, Wen G, Butzke D, Platzer M, Fusetani N, Matsunaga S: Antitumour polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei. Proc Natl Acad Sci USA 2004b;101:16222–16227.
  78. Podar M, Eads JR, Richardson TH: Evolution of a microbial nitrilase gene family: a comparative and environmental genomics study. BMC Evol Biol 2005;5:42.
  79. Purcarea C, Herve G, Cunin R, Evans DR: Cloning, expression, and structure analysis of carbamate kinase-like carbamoyl phosphate synthetase from Pyrococcus abyssi. Extremophiles 2001;5:229–239.
  80. Ragauskas AJ, Williams CK, Davidson BH, Britovsek G, Cairney J, Eckert CA, Frederick WJ Jr, Hallett JP, Leak DJ, Liotta CL, Mielenz JR, Murphy R, Templer R, Tschaplinski T: The path forward for biofuels and biomaterials. Science 2006;311:484–489.
  81. Ranjan R, Grover A, Kapardar RK, Sharma R: Isolation of novel lipolytic genes from uncultured bacteria of pond water. Biochem Biophys Res Commun 2005;335:57–65.
  82. Rees HC, Grant S, Jones B, Grant WD, Heaphy S: Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries. Extremophiles 2003;7:415–421.
  83. Rhee J-K, Ahn D-G, Kim Y-G, Oh J-W: New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library. Appl Environ Microbiol 2005;71:817–825.
  84. Richardon TH, Tan X, Frey G, Callen W, Cabell M, Lam D, Macomber J, Short JM, Robertson DE, Miller C: A novel, high-performance enzyme for starch liquefaction. J Biol Chem 2002;277:26501–26507.
  85. Riesenfeld CS, Goodman RM, Handelsman J: Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environ Microbiol 2004;6:981–989.
  86. Robertson DE, Chaplin JA, DeSantis G, Podar M, Madden M, Chi E, Richardson T, Milan A, Miller M, Weiner DP, Wong K, McQuaid J, Farwell B, Preston LA, Tan X, Snead MA, Keller M, Mathur E, Kretz PL, Burk MJ, Short JM: Exploring nitrilase sequence space for enantioselective catalysis. Appl Environ Microbiol 2004;70:2429–2436.
  87. Rondon MR, August PR, Bettermann AD, Brady SF, Grossman TH, Liles MR, Loiacono KA, Lynch BA, MacNeil IA, Minor C, Tiong CL, Gilman M, Osburne MS, Clardy J, Handelsman J, Goodman RM: Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl Environ Microbiol 2000;66:2541–2547.
  88. Rothschild LJ, Mancinelli RL: Life in extreme environments. Nature 2001;409:1092–1101.
  89. Schirmer A, Gadkari R, Reeves CD, Ibrahim F, DeLong EF, Hutchinson CR: Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta. Appl Environ Microbiol 2005;71:4840–4849.
  90. Schmeisser C, Stöckigt C, Raasch C, Wingender J, Timmis KN, Wenderoth DF, Flemming H-C, Liesegang H, Schmitz RA, Jaeger K-E, Streit WR: Metagenome survey of biofilms in drinking-water networks. Appl Environ Microbiol 2003;69:7298–7309.
  91. Schmidt TM, DeLong EF, Pace NR: Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing. J Bacteriol 1991;173:4371–4378.
  92. Schwarz S, Waschkowitz T, Daniel R: Enhancement of gene detection frequencies by combining DNA-based stable-isotope probing with the construction of metagenomic DNA libraries. World J Microbiol Biotechnol 2006;22:363–367.
  93. Steele HL, Streit WR: Metagenomics: advances in ecology and biotechnology. FEMS Microbiol Lett 2005;247:105–111.
  94. Streit WR, Daniel R, Jaeger K-E: Prospecting for biocatalysts and drugs in the genomes of non-cultured microorganisms. Curr Opin Biotechnol 2004;15:285–290.
  95. Streit WR, Schmitz RA: Metagenomics: the key to uncultured microbes. Curr Opin Microbiol 2004;7:492–498.
  96. Tang K, Utairungsee T, Kanokratana P, Sripang R, Champreda V, Eurwilaichitr L, Tanapongpipat S: Characterisation of a novel cyclomaltodextrinase expressed from environmental DNA isolated from Bor Khleung hot spring in Thailand. FEMS Microbiol Lett 2006;260:91–99
  97. Tsai YL, Olson BH: Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Appl Environ Microbiol 1992;58:2292–2295.
  98. Uchiyama T, Abe T, Ikemura T, Watanabe K: Substrate induced gene expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat Biotechnol 2005;23:88–93.
  99. Van Sint Fiet S, van Beilen JB, Witholt B: Selection of biocatalysts for chemical synthesis. Proc Natl Acad Sci USA 2006;103:1693–1698.
  100. Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KA, Nelson W, Fouts DE, Levy S, Knap AH, Lomas MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers Y-H, Smith HO: Environmental genome shotgun sequencing of the Sargasso Sea. Science 2004;304:66–74.
  101. Voget S, Leggewie C, Uesbeck A, Raasch C, Jaeger K-E, Streit WR: Prospecting for novel biocatalysts in a soil metagenome. Appl Environ Microbiol 2003;69:6235–6242.
  102. Voget S, Steele HL, Streit, WR: Characterisation of a metagenome-derived halotolerant cellulase. J Biotechnol 2006;126:26–36.
  103. Wang C, Meek DJ, Panchal P, Boruvka N, Archibald FS, Driscoll BT, Charles TC: Isolation of poly-4-hydroxybutyrate metabolism genes from complex microbial communities by phenotypic complementation of bacterial mutants. Appl Environ Microbiol 2006a;72:384–391.
  104. Wang G: Diversity and biotechnological potential of the sponge-associated microbial consortia. J Ind Microbiol Biotechnol 2006b;33:545–551.
  105. Wang G-Y-S, Graziani E, Waters B, Pan W, Li X, McDermott J, Meurer G, Saxena G, Andersen RJ, Davies J: Novel natural products from soil DNA libraries in a streptomycete host. Org Lett 2000;2:2401–2404.
  106. Wenzel SC, Müller R: Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways. Curr Opin Biotechnol 2005;16:594–606.
  107. Yoon, S, Kim S, Ryu Y, Kim TD: Identification and characterization of a novel (S)-ketoprofen-specific esterase. Int J Biol Macromol 2007; 41:1–7.
  108. Yun J, Kang S, Park S, Yoon H, Kim M-J, Heu S, Ryu S: Characterization of a novel amylolytic enzyme encoded by a gene from a soil-derived metagenomic library. Appl Environ Microbiol 2004;70:7229–7235.
  109. Yun J, Ryu S: Screening for novel enzymes from metagenome and SIGEX, as a way to improve it. Microb Cell Fact 2005;4:8.

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Published online: October 29, 2008
Issue release date: October 2008

Number of Print Pages: 13
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ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

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