Cells Tissues Organs
Immunocytochemical Demonstration of Two Vitamin D-Dependent Calcium-Binding Proteins in Mammalian KidneySchreiner D.S.a · Jande S.S.a · Parkes C.O.b · Lawson D.E.M.c · Thomasset M.daDepartment of Anatomy, University of Ottawa, Ont., Canada; bDepartment of Physiology, University of British Columbia, Vancouver, B.C., Canada; cDunn Nutritional Laboratory, University of Cambridge and Medical Research Council, Cambridge, England; dInserm, U.120, Le Vésinet, France
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Article / Publication Details
Received: December 05, 1982
Accepted: January 04, 1983
Published online: July 11, 2008
Issue release date: 1983
Number of Print Pages: 14
Number of Figures: 0
Number of Tables: 0
ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)
For additional information: https://www.karger.com/CTO
Abstract
Distribution of vitamin D-dependent calcium-binding proteins (CaBPs) were studied in four mammalian species using monospecific antibodies raised against chick duodenal CaBP (D-CaBP), human cerebellar CaBP (L-CaBP), and rat duodenal CaBP (S-CaBP). The immunoperoxidase technique of unlabelled antibodies was employed. The distribution of D-CaBP/L-CaBP was identical in all the species studied except for the monkey, in the rat, pig, and human nephrons, D-CaBP/L-CaBP was seen in the cytoplasm of the cells of the distal convoluted tubules, initial segments of the collecting ducts and interspersed cells of the collecting ducts. Proximal convoluted tubules, glomeruli and maculae densae were negative. In the monkey, in addition to the cells of the distal convoluted tubules, the cells along the entire length of the collecting ducts were also strongly positive. S-CaBP was found to be species-specific, and hence positive results were obtained only in the rat nephron. The strongest positive reaction for S-CaBP was seen in the cells of the distal convoluted tubules. These same cells were also positive for D-CaBP/L-CaBP. S-CaBP was also detected in the cells of the thick ascending limb of the loop of Henle, along the entire length of the collecting ducts and in smaller amounts in cells of the macula densa. Intracellularly the S-CaBP was present only in the apical cytoplasm of positive cells. D-CaBP/L-CaBP stained the entire cytoplasm but the staining in the apical cytoplasm was denser.
© 1983 S. Karger AG, Basel
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Article / Publication Details
Received: December 05, 1982
Accepted: January 04, 1983
Published online: July 11, 2008
Issue release date: 1983
Number of Print Pages: 14
Number of Figures: 0
Number of Tables: 0
ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)
For additional information: https://www.karger.com/CTO
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