Cells Tissues Organs

 

Electron Probe X-Ray Microanalysis of Calcium and Phosphorus Distribution in Developing Hamster Tooth Germs in vitro and in vivo

Stratmann U.a · Barckhaus R.H.b · Lyaruu D.M.c · Wöltgens J.H.M.c · Wessling G.d · Baumeister A.a

Author affiliations

aInstitute of Anatomy, bInstitute of Medical Physics and dSchool of Dentistry, University of Münster, FRG; cLaboratory of Tooth Development, Department Oral Cell Biology, Academic Centre for Dentistry, Vrije Universiteit, Amsterdam, The Netherlands

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Acta Anat 1991;140:343–349

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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: May 15, 1990
Accepted: October 23, 1990
Published online: July 16, 2008
Issue release date: 1991

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO

Abstract

The aim of the present study was to investigate the spatial distribution of Ca and P in dentin and enamel of developing first (M1) and second (M2) maxillary hamster molars (age: 3–5 days) in comparison with cultured molars. For culturing the germs were dissected from 3-day-old hamsters and incubated for 1 and 2 days, respectively, in a modified BGJb medium. Electron probe X-ray measurements were carried out on 3 regions extending in a vertical axis from cusp tip over cusp middle to cusp base next to the cervical loop region. Neither the in vivo nor the in vitro group was statistically different in the Ca and P concentration in the regions of dentin. In both groups the measurements in enamel showed a gradient with an increase in Ca and P from enamel surface towards dentin-enamel junction and a gradient with an increase from cusp base towards cusp tip. Direct comparison of the in vivo group with the in vitro group did not demonstrate a statistical difference between the mineral content of the 4-day-old germs and the 1-day culture germs, respectively the 5-day-old germs and the 2-day culture germs. The results indicate a high correspondence between the mineralization process of in vitro and in vivo tooth germ development.

© 1991 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: May 15, 1990
Accepted: October 23, 1990
Published online: July 16, 2008
Issue release date: 1991

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO


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