Quinolone Resistance and Correlation to Other Antimicrobial Resistances in Faecal Isolates of Escherichia coli in Hong KongChu Y.W. · Cheung T.K.M. · Wong C.H. · Tsang G.K.L. · Lee K. · Lau S.S.Y. · Kam K.M.
Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong, SAR, China
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Article / Publication Details
In an attempt to assess the level of quinolone resistance and its association with other antimicrobial resistance in faecal Escherichia coli isolated from routine outpatient specimens in Hong Kong, ciprofloxacin-supplemented MacConkey agar was used to screen for resistant isolates. Antimicrobial susceptibility testing of the isolates was done by VITEK 2 and previous amplification-based methods were employed to characterize the genetic determinants behind some of the resistance phenotypes. One hundred and seventy-six (43%) of 409 specimens had quinolone-resistant E. coli isolated (199 isolates). Quinolone resistance was found to be associated with resistances to penicillins (>80%) and co-trimoxazole (69%). Nonsusceptibility to combinations of penicillins and clavulanic acid was above 20% and up to 50% for the aminoglycosides gentamicin and tobramycin. CTX-M-type extended-spectrum β-lactamases were found responsible for most cephalosporin resistances but the transferable quinolone resistance determinant qnrA was not detected. Our data suggested that a high percentage of E. coli isolates as part of the alleged normal intestinal microflora in humans appeared to be resistant to quinolones. Co-resistance to various other frequently used antimicrobials was also observed. Transferable genetic determinants were found to be involved in some cases.
© 2008 S. Karger AG, Basel
- Ling TK, Liu EY, Cheng AF: A 13-year study of antimicrobial susceptibility of common gram-negative bacteria isolated from the bloodstream in a teaching hospital. Chemotherapy 2001;47:29–38.
- Kam KM, Kam SSY, Cheung DTL, Tung VWN, Au WF, Cheung MM: Molecular characterization of quinolone-resistant Neisseria gonorrhoeae in Hong Kong. Antimicrob Agents Chemother 2003;47:436–439.
- Chu YW, Chu MY, Luey KY, Ngan YW, Tsang KL, Kam KM: Genetic relatedness and quinolone resistance of Campylobacter jejuni strains isolated in 2002 in Hong Kong. J Clin Microbiol 2004;42:3321–3323.
- Lee CC, Lee VW, Chan FK, Ling TK: Levofloxacin-resistant Helicobacter pylori in Hong Kong. Chemotherapy 2008;54:50–53.
- Cheung TKM, Chu MY, Ma CH, Yung RWH, Kam KM: Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype Enteritidis in Hong Kong. J Antimicrob Chemother 2005;56:586–589.
- Qin X, Razia Y, Johnson JR, Stapp JR, Boster DR, Tsosie T, Smith DL, Braden C, Gay K, Angulo FJ, Tarr PI: Ciprofloxacin-resistant gram-negative bacilli in the faecal microflora of children. Antimicrob Agents Chemother 2006;50:3325–3329.
CLSI: Performance Standards for Antimicrobial Susceptibility Testing. Supplement M100-S13. Wayne, Clinical and Laboratory Standard Institute, 2003.
- Hooper DC: Mechanisms of quinolone resistance. Drug Resist Updates 1999;2:38–55.
- Philippon A, Arlet G, Jacoby GA: Plasmid-determined AmpC-type β-lactamases. Antimicrob Agents Chemother 2002;46:1–11.
- Kadima TA, Weiner JH: Mechanism of suppression of piperacillin resistance in Enterobacteria by tazobactam. Antimicrob Agents Chemother 1997;41:2177–2183.
- Leverstein-van Hall MA, Blok HEM, Donders ART, Paauw A, Fluit AC, Verhoef J: Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. J Infect Dis 2003;187:251–259.
- Lautenbach E, Strom BL, Bilker WB, Patel JB, Edelstein PH, Fishman NO: Epidemiological investigation of fluoroquinolone resistance in infections due to extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Clin Infect Dis 2001;33:1288–1294.
- Karlowsky JA, Thornsberry C, Jones ME, Sahm DF: Susceptibility of antimicrobial-resistant urinary Escherichia coli isolates to fluoroquinolones and nitrofurantoin. Clin Infect Dis 2003;36:183–187.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.