A Randomized Double-Blind, Placebo-Controlled Study to Establish the Effects of Spirulina in Elderly KoreansPark H.J.a · Lee Y.J.b · Ryu H.K.b · Kim M.H.c · Chung H.W.d · Kim W.Y.b
aFoods R&D, CJ CheilJedang Corp., Seoul, bNutritional Science and Food Services, Ewha Womans University, Seoul, cFood Science and Nutrition, Kyungpook National University, Daegu, and dObstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea
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Aims: This study was conducted to determine the antioxidant capacity, immunomodulatory and lipid-lowering effects of spirulina in healthy elderly subjects and to document the effectiveness of spirulina as a functional food for the elderly. Methods: A randomized double-blind, placebo-controlled study was performed. The subjects were 78 individuals aged 60–87 years and were randomly assigned in a blinded fashion to receive either spirulina or placebo. The elderly were instructed to consume the spirulina or placebo at home, 8 g/day, for 16 consecutive weeks. Results: In male subjects, a significant plasma cholesterol-lowering effect was observed after the spirulina intervention (p < 0.05). Spirulina supplementation resulted in a significant rise in plasma interleukin (IL)-2 concentration, and a significant reduction in IL-6 concentration. A significant time-by-treatment intervention for total antioxidant status was observed between spirulina and placebo groups (p < 0.05). In female subjects, significant increases in IL-2 level and superoxide dismutase activity were observed (p < 0.05) after spirulina supplementation. There were significant reductions in total cholesterol in female subjects. Conclusions: The results demonstrate that spirulina has favorable effects on lipid profiles, immune variables, and antioxidant capacity in healthy, elderly male and female subjects and is suitable as a functional food.
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- Belay A, Ota Y, Miyakawa K, Shimamatsu H: Current knowledge on potential health benefits of spirulina. J Appl Phycol 1993;5:235–241.
- Salazar M, Chamorro G, Salazar S, Steele C: Effect of Spirulina maxima consumption on reproductive and peri- and postnatal development in rats. Food Chem Toxicol 1996;34:353–359.
- Chamorro G, Salazar S, Castillo L, Steele C, Salazar M: Reproductive and peri- and postnatal evaluation of Spirulina maxima in mice. J Appl Phycol 1997;9:107–112.
- Salazar M, Martinez E, Madrigal E, Ruiz LE, Chamorro G: Subchronic toxicity study in mice fed Spirulina maxima. J Ethnopharmacol 1998;62:235–241.
Kato T, Takemoto K, Katayama H, Kuwabara Y: Effects of spirulina (Spirulina platensis) on dietary hypercholesterolemia in rats. J Jap Soc Nutr Food Sci 1984;37:323–332.
Ishii K, Katoh T, Okuwaki Y, Hayashi O: Influence of dietary Spirulina platensis on IgA level in human saliva. J Kagawa Nutr Univ 1999;30:27–33.
Qureshi MA, Kidd MT, Ali RA: Spirulina platensis extract enhances chicken macrophage functions after in vitro exposure. J Nutr Immunol 1995;3:35–44.
Dainippon Ink & Chemicals, Inc (DIC): Antitumoral agents containing phycobilin. Japanese Patent #58-65216, 1983.
- Pinero Estrada JE, Bermejo Descos P, Villar dell Fresno AM: Antioxidant activity of different fractions of Spirulina platensis extract. Farmaco 2001;56:497–500.
- Hirata T, Tanaka M, Ooike M, Tsunomura T, Sakaguchi M: Antioxidant activities of phycocyanobilin prepared from Spirulina platensis. J Appl Phycol 2000;12:435–439.
- Mani UV, Desai S, Iyer UM: Studies on the long-term effect of spirulina supplementation on serum lipid profile and glycated proteins in NIDDM patients. J Nutraceut Funct Med Foods 2000;2:25–32.
- Benedetti S, Benvenuti F, Pagliarani S, Francogli S, Scoglio S, Canestrari F: Antioxidant properties of a novel phycocyanin extract from the blue-green alga Aphanizomenon flos-aquae. Life Sci 2004;75:2353–2362.
- Bhat VB, Madyastha KM: C-phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem Biophys Res Commun 2000;1:20–25.
- Hayashi O, Katoh T, Okuwaki Y: Enhancement of antibody production in mice by dietary Spirulina platensis. J Nutr Sci Vitaminol 1994;40:431–441.
- Voss P, Siems W: Clinical oxidation parameters of aging. Free Radic Res 2006;40:1339–1349.
- Lelliott C, Vidal-Puig AJ: Lipotoxicity, an imbalance between lipogenesis de novo and fatty acid oxidation. Int J Obes Relat Metab Disord 2004;28:22–28.
- Vasto S, Candore G, Balistreri CR, Caruso M, Colonna-Romano G, Grimaldi MP, Listi F, Nuzzo D, Lio D, Caruso C: Inflammatory networks in aging, age-related disease and longevity. Mech Ageing Dev 2007;128:83–91.
- Parikh P, Mani U, Iyer UM: Role of spirulina in the control of glycemia and lipidemia in type 2 diabetes mellitus. J Med Food 2001;4:193–199.
- Romana I, Bellitti MR, Nicolaus DD, Lama-Lica-Manca MC: Lipid profile, a useful chemotaxonomic marker for classification of a new cyanobacterium in Spirulina genus. Phytochem 2000;54:289–296.
- Watanabe F, Takenaka S, Kittaka-Katsura H, Ebara S, Miyamoto E: Characterization and bioavailability of vitamin B12 compounds from edible algae. J Nutr Sci Vitaminol 2002;48:325–331.
- Kuhad A, Tirkey N, Pilkhwal S, Chopra K: Effect of spirulina, a blue green algae, on gentamicin-induced oxidative stress and renal dysfunction in rats. Fund Clin Pharmacol 2006;20:121–128.
- Samuels R, Mani UV, Iyer UM, Nagaak US: Hypocholesterolemic effects of spirulina in patients with hyperlipidemic nephritic syndrome. J Med Food 2002;5:91–96.
- Nagaoka S, Shimizu K, Kaneko H, Shibayama F, Morikawa K, Kanamaru Y, Otsuka A, Hirahashi T, Kato T: A novel protein C-phycocyanin plays a role in the hypocholesterolemic action of Spirulina platensis concentrate in rats. J Nutr 2005;135:2425–2430.
Korean Nutrition Society: Nutrition Information Center. Nutritional Assessment Program CAN pro 3.0, 2006.
- Friedewald WT, Levy RI, Fredrickson DS: Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502.
- Lauer RM, Lee J, Clarke WR: Factors affecting the relationship between childhood and adult cholesterol levels: the Muscatine Study. Pediatrics 1988;82:309–318.
Yagi K: Assay for blood plasma or serum; in Methods in Enzymology. New York, Academic Press, 1984, vol 105, pp 328–331.
- Marklund S, Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469–474.
- Sheri ZC, Keen CL, Hurley LS: Superoxide dismutase activity and lipid peroxidation in the rat: developmental correlation effected by manganese deficiency. J Nutr 1983;113:2498–2504.
- Kim WY, Park JY: The effects of spirulina on lipid metabolism, antioxidant capacity and immune function in Korean elderly. Kor J Nutr 2003;36:287–297.
- Nakaya N, Homma Y, Goto Y: Cholesterol-lowering effect of spirulina. Nutr Rep Int 1988;37:1329–1337.
- De Rivera C, Miranda-Zamora R, Diaz-Zagoya JC, Oropeza M: Preventive effect of Spirulina maxima on the fatty liver induced by a fructose-rich diet in the rat. Life Sci 1993;53:57–61.
- Hosoyamada Y, Takai T, Kato T: Effects of water-soluble and insoluble fractions of spirulina on serum lipid components and glucose tolerance in rats. J Jpn Soc Nutr Food Sci 1991;44:273–277.
- Miranda MS, Cintra R, Barros SBM, Mancini-Filho J: Antioxidant activity of the microalga Spirulina maxima. Braz J Med Biol Res 1998;31:1075–1079.
- Mao TK, van de Water J, Gershwin ME: Effect of spirulina on the secretion of cytokines from peripheral blood mononuclear cells. J Med Food 2000;3:135–140.
- Adler WH 3rd: Immune function in the elderly. Geriatrics 1989:44(suppl A):7–10.
Thoman ML, Weigle WO: Lymphokines and aging: interleukin-2 production and activity in aged animals. J Immunol 1981:127:102–106.
- Rabinowich H, Goses Y, Reshef T, Klajman A: Interleukin-2 production and activity in aged humans. Mech Ageing Dev 1985:32:2113–216.
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