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Original Paper

Expression of Tissue Factor by Eosinophils in Patients with Chronic Urticaria

Cugno M.a · Marzano A.V.b · Tedeschi A.c · Fanoni D.b · Venegoni L.b · Asero R.d

Author affiliations

aDepartment of Internal Medicine, bInstitute of Dermatological Sciences, cAllergy and Clinical Immunology Unit, IRCCS Foundation Maggiore Policlinico Hospital, Mangiagalli, Regina Elena and University of Milan, and dAmbulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, Milan, Italy

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Int Arch Allergy Immunol 2009;148:170–174

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 15, 2008
Accepted: April 23, 2008
Published online: September 19, 2008
Issue release date: January 2009

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: Although several cases of chronic urticaria (CU) are currently regarded as autoimmune in origin, associated with histamine-releasing autoantibodies, an activation of blood coagulation via tissue factor (TF) and a strong expression of TF in lesional skin have been described. Eosinophils, which are involved in CU skin lesions, have recently been demonstrated as the major source of TF in human blood. We assessed whether eosinophils are the cellular source of TF in CU skin lesions. Methods: Twenty patients with severe CU were studied. Skin biopsy specimens were taken from wheals. The control group consisted of specimens of perilesional normal skin from different types of skin tumours (10) and various skin disorders with non-eosinophilic infiltrates, including leukocytoclastic vasculitis (7), lichen planus (8) and mastocytosis (3). TF expression was evaluated by immunohistochemical methods using an anti-TF monoclonal antibody. Co-localization of TF and eosinophil cationic protein, a classic cell marker of eosinophils, was investigated by double-staining studies using 2 specific monoclonal antibodies in the 4 specimens showing the highest TF reactivity scores. Results: All specimens from patients with CU clearly showed TF expression that was absent in all normal control specimens (p = 0.0001) and in the skin disorders with non-eosinophilic infiltrates (p = 0.001–0.0001). The double-staining experiments for TF and eosinophil cationic protein clearly showed that the TF-positive cells were eosinophils. Conclusions: Eosinophils are the main source of TF in CU lesional skin. This finding highlights the role of these cells in the pathophysiology of CU and might pave the way for new therapeutic strategies.

© 2008 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 15, 2008
Accepted: April 23, 2008
Published online: September 19, 2008
Issue release date: January 2009

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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