The human UBE2L6 gene encodes UbcH8Kumar, a ubiquitin-conjugating enzyme (E2) highly simliar in primary structure to UbcH7 which is encoded by UBE2L3. Like UBC4 and UBC5 in yeast, these proteins demonstrate functional redundancy. Herein we report the intron/exon structure of UBE2L6. Comparison of the genomic organization of UBE2L6 with UBE2L3 demonstrates that these genes remain highly conserved at the genomic as well as at the protein level. We also describe the chromosomal localization of UBE2L6, which maps to chromosome 11q12.    

1.
Ardley HC, Moynihan TP, Thompson J, Leek JP, Markham AF, Robinson PA: Rapid isolation of genomic clones for individual members of human multigene families: Identification and localisation of UBE2L4, a novel member of a ubiquitin conjugating enzyme dispersed gene family. Cytogenet Cell Genet 79:188–192 (1997).
2.
Harbers K, Muller U, Grams A, Li E, Jaenisch R, Franz, T: Provirus integration into a gene encoding a ubiquitin-conjugating enzyme results in a placental defect and embryonic lethality. Proc natl Acad Sci, USA 93:12412–12417 (1996).
3.
Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM: Identification of a family of closely related human ubiquitin-conjugating enzymes. J biol Chem 270:30408–30414 (1995).
4.
Kumar S, Kao WH, Howley PM: Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity. J biol Chem 272:13548–13554 (1997).
5.
Matuschewski K, Hauser H-P, Treier M, Jentsch S: Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions. J biol Chem 271:2789–2791 (1996).
6.
Moynihan TP, Ardley HC, Leek JP, Thompson J, Brindle NS, Markham AF, Robinson PA: Characterization of a human ubiquitin-conjugating enzyme gene, UBE2L3. Mammal Genome 7:520–525 (1996).
7.
Moynihan TP, Cole CG, Dunham I, Markham AF, Robinson PA: Fine-mapping, genomic structure determination, and transcript analysis of the human ubiquitin-conjugating enzyme gene UBE2L3. Genomics 51:124–127 (1998).
8.
Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Scheffner M, Markham AF, Robinson PA: RING finger and IBR motifs characterize the interaction domains of HHARI and H7-AP1 with the ubiquitin-conjugating enzymes UbcH7 and UbcH8. J biol Chem 274:30963–30968 (1999).
9.
Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M: Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J biol Chem 271:2795–2800 (1996).
10.
Robinson PA, Leek JP, Thompson J, Carr IM, Bailey A, Moynihan TP, Coletta PL, Lench NJ, Markham AF: A human ubiquitin-conjugating enzyme, L-UBC, maps in the Alzheimer’s disease locus on chromosome 14q24.3. Mammal Genome 6:725–731 (1995).
11.
Scheffner M, Huibregtse JM, Howley PM: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Proc natl Acad Sci, USA 91:8797–8801 (1994).
© 2000 S. Karger AG, Basel
2000
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.