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An anchored YAC-STS framework for the rat genome

Cai L.a,b · Lindpaintner K.a-c · Browne J.d · Gruetzner F.e · Haaf T.e · James M.R.d · Bihoreau M.-T.d

Author affiliations

aCardiovascular Division, Brigham and Women’s Hospital, Department of Cardiology, Children’s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA (USA); bF. Hoffmann-La Roche Ltd, Roche Genetics, Pharmaceuticals Division, Basel (Switzerland); cMax Delbrück Center for Molecular Medicine, Berlin (Germany); dThe Wellcome Trust Center for Human Genetics, University of Oxford, Oxford (UK); and eMax Planck Institute for Molecular Genetics, Berlin-Dahlem (Germany)

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Cytogenet Cell Genet 89:168–170 (2000)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: August 07, 2000
Issue release date: 2000

Number of Print Pages: 3
Number of Figures: 1
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Abstract.

We report here the first YAC-STS framework for the rat genome. A total of 417 anchor microsatellite markers were used to screen a 10-fold redundant YAC library. One or more unambiguous YACs were identified for 372 markers. Assuming the genetic length of the rat genome to be 2,000 cM (Bihoreau et al. 1997b), the YAC-STS framework will provide, on average, one informative YAC clone every 5.4 cM. A total of 111 anchor markers used in this study were derived from known gene regions. We also demonstrated one of the important and immediate uses of this YAC-STS framework, which is to establish a correlation between the genetic and cytogenetic maps in the rat through FISH analysis.   

© 2000 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: August 07, 2000
Issue release date: 2000

Number of Print Pages: 3
Number of Figures: 1
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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