Gerontology

Regenerative and Technological Section

Free Access

lin– Sca-1+ Cells and Age-Dependent Changes of Their Proliferation Potential Are Reliant on Mesenchymal Stromal Cells and Are Leukemia Inhibitory Factor Dependent

Schraml E.a, b · Fehrer C.c · Brunauer R.c · Lepperdinger G.c · Chesnokova V.d · Schauenstein K.a, †

Author affiliations

aInstitute of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, Graz, bInstitute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, and cInstitute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria; dCedars-Sinai Medical Center, Los Angeles, Calif., USA

Corresponding Author

Günter Lepperdinger

Institute for Biomedical Aging Research, Austrian Academy of Sciences

Rennweg 10, AT–6020 Innsbruck (Austria)

Tel. +43 512 5839 1934, Fax +43 512 5839 198

E-Mail guenter.lepperdinger@oeaw.ac.at

Keywords: Hematopoietic stem and progenitor cellsMesenchymal stromal cellsLeukemia inhibitory factorSenescence evasion factor

Related Articles for ""
Gerontology 2008;54:312–323
https://doi.org/10.1159/000161736

Abstract

Aging as a process is paralleled by a variety of hematological alterations. Characteristic features are a diminished homeostatic control of blood cell production and a decline in immune functions. It is generally accepted that stromal cells play a basal role in hematopoiesis by providing survival and differentiation signals, by secreting cytokines, or through direct contact with hematopoietic stem cells, thereby supporting the generation and replenishment of hematopoi- etic progenitor cells (HPC). Here we demonstrated that HPC-related colony formation is positively influenced by mesenchymal stromal cells (MSCs) when grown in co-culture, in particular regarding the number of primary granulocyte/macrophage colony-forming units as well as with respect to the average size of the formed colonies. These effects were more pronounced when the MSCs originated from young donors than from old ones. Because leukemia inhibitory factor (LIF) plays an important role during hematopoiesis, properties of lin– Sca-1+ cells and MSCs derived from LIF-deficient mice (LIF–/–) were determined both ex vivo and in vitro. LIF–/– animals contain a significantly reduced number of lin– Sca-1+ cells, nevertheless the replating capacity of LIF–/– HPCs was found to be generally unchanged when compared to those from LIF+/+ animals. However, when cocultured with MSCs, LIF–/– lin– Sca-1+ cells exhibited comparable characteristics to HPCs derived from old wild-type animals.

© 2008 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Regenerative and Technological Section

Received: March 11, 2008
Accepted: July 28, 2008
Published online: October 08, 2008
Issue release date: October 2008

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 1

ISSN: 0304-324X (Print)
eISSN: 1423-0003 (Online)

For additional information: https://www.karger.com/GER

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