Cellular Physiology and Biochemistry
Original Paper
Intracellular pH Regulation in Bovine Aortic Endothelial Cells: Evidence of Both Na+/H+ Exchange and Na+-Dependent Cl–/HCO–3 ExchangeFaber S.a · Lang H.-J.a · Hock F.J.a · Schölkens B.A.a · Mutschler E.ba Hoechst Marion Roussel Deutschland GmbH, and b Department of Pharmacology, University Frankfurt/Main, Germany
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Cell Physiol Biochem 1998;8:202–211
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Article / Publication Details
First-Page Preview
Published online: July 03, 1998
Issue release date: 1998
Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)
For additional information: https://www.karger.com/CPB
Abstract
Regulation of intracellular pH (pHi) was studied in cultured bovine aortic endothelial cells, an important cell system for cardiovascular research. Suspended cells were acidified by the NH4Cl prepulse technique as well as by exposure to CO2/HCO–3. Subsequent rates of pHi recovery were monitored using the fluorescent dye 2′,7′-bis(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). In HCO–3-free solutions, an EIPA-sensitive, Na+-dependent mechanism fully accounted for realkalinization, namely the Na+/H+ exchanger (NHE). In the presence of HCO–3, an additional acid efflux mechanism was found. This one was dependent on Na+ and intracellular Cl–, EIPA-insensitive but DIDS-sensitive, and therefore represented a Na+-dependent Cl–/HCO–3 exchanger (NCBE). In summary, two acid-extruding mechanisms were identified in bovine aortic endothelial cells: NHE and NCBE.
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References
- Murer H, Hopfer U, Kinne R: Sodium/proton antiport in brush-border membrane vesicles isolated from rat small intestine and kidney. Biochem J 1976;154:597–604.
- Mahnensmith RL, Aronson PS: The plasma membrane sodium-hydrogen exchanger and its role in physiological and pathophysiological processes. Circ Res 1985;56:773–788.
-
Vigne P, Frelin C, Cragoe EJ Jr, Lazdunski M: Structure-activity relationships of amiloride and certain of its analogues in relation to the blockade of the Na+/H+ exchange system. Mol Pharmacol 1983;25:131–136.
-
Faber S, Lang HJ, Scholz W, Weichert A, Hock FJ, Schölkens BA, Mutschler E: Inhibition of Na+/H+ exchange in bovine aortic endothelial cells by novel carbonylguanidine derivatives. Drug Res (submitted).
- Wakabayashi S, Shigekawa M, Pouysségur J: Molecular physiology of vertebrate Na+/H+ exchangers. Physiol Rev 1997;77:51–74.
- Madshus IH: Regulation of intracellular pH in eukaryotic cells. Biochem J 1988;250:1–8.
- Reinertsen KV, Tønnessen TI, Jacobsen J, Sandvig K, Olsnes S: Role of chloride/bicarbonate antiport in the control of cytosolic pH. J Biol Chem 1988;263:11117–11125.
- Cabantchik ZI, Rothstein A: The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol 1972;10:311–330.
-
Faber S, Lang HJ, Hropot M, Schölkens BA, Mutschler E: A novel screening assay of the Na+-dependent Cl–/HCO–3 exchanger (NCBE) and its inhibitors. Cell Physiol Biochem 1996;6:39–49.
- Li K, Sirois P, Rouleau JL: Role of endothelial cells in cardiovascular function. Life Sci 1994;54:579–592.
-
Faber S, Lang HJ, Hock FJ, Schölkens BA, Mutschler E: Intracellular pH regulation in bovine aortic endothelial cells: Evidence of both Na+/H+ exchange and Na+-dependent Cl–/HCO–3 exchange. Pflügers Arch 1997;433:R112.
External Resources
-
Roos A, Boron WF: Intracellular pH. Physiol Rev 1981;61:294–434.
- Vigne P, Frelin C, Cragoe EJ Jr, Lazdunski M: Ethylisopropyl-amiloride: A new and highly potent derivative of amiloride for the inhibition of the Na+/H+ exchange in various cell types. Biochem Biophys Res Commun 1983;116:86–90.
- Lepke S, Fasold H, Pring M, Passow H: A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS). J Membr Biol 1976;29:147–177.
- Kahn AM, Cragoe EJ Jr, Allen JC, Halligan RD, Shelat H: Na+-H+ and Na+-dependent Cl–/HCO–3 exchange control pHi in vascular smooth muscle. Am J Physiol 1990;259:C134–C143.
-
Jaffe EA: Biology of Endothelial Cells. Boston, Nijhoff, 1984.
- Bussolino F, Wang JM, Turrini F, Alessi D, Ghigo D, Costamagna C, Pescarmona G, Mantovani A, Bosia A: Stimulation of the Na+/H+ exchanger in human endothelial cells activated by granulocyte- and granulocyte-macrophage-colony-stimulating factor. J Biol Chem 1989;264:18284–18287.
- Escobales N, Longo E, Cragoe EJ Jr, Danthuluri NR, Brock TA: Osmotic activation of Na+-H+ exchange in human endothelial cells. Am J Physiol 1990;259:C640–C646.
- Ghigo D, Bussolino F, Garbarino G, Heller R, Turrini F, Pescarmona G, Cragoe EJ Jr, Pegoraro L, Bosia A: Role of Na+/H+ exchange in thrombin-induced platelet-activating factor production by human endothelial cells. J Biol Chem 1988;263:19437–19446.
- Kitazono T, Takeshige K, Cragoe EJ Jr, Minakami S: Intracellular pH changes of cultured bovine aortic endothelial cells in response to ATP addition. Biochem Biophys Res Commun 1988;152:1304–1309.
- Bonanno J, Giasson C: Intracellular pH regulation in fresh and cultured bovine corneal endothelium. I. Na+/H+ exchange in the absence and presence of HCO–3. Invest Ophthalmol Vis Sci 1992;33:3058–3067.
- Bonanno J, Giasson C: Intracellular pH regulation in fresh and cultured bovine corneal endothelium. II. Na+:HCO–3 cotransport and Cl–/ HCO–3 exchange. Invest Ophthalmol Vis Sci 1992;33:3068–3079.
- Chu TC, Keith CH, Yee E, Green K: Intracellular pH of tissue-cultured bovine corneal endothelial cells. Ophthalmic Res 1992;24:265–273.
- Jentsch TJ, Korbmacher C, Janicke I, Fischer DG, Stahl F, Helbig H, Hollwede H, Cragoe EJ Jr, Keller SK, Wiederholt M: Regulation of cytoplasmic pH of cultured bovine corneal endothelial cells in the absence and presence of bicarbonate. J Membr Biol 1988;103:29–40.
- Peral MJ, Calonge ML, Ilundáin AA: Na+-HCO–3 cotransporter and intracellular pH regulation in chicken enterocytes. Pflügers Arch 1995;430:612–616.
- Townsley MC, Machen TE: Na-HCO3 cotransport in rabbit parietal cells. Am J Physiol 1989;257:G350–G356.
- Kikeri D, Azar S, Sun A, Zeidel ML, Hebert SC: Na+-H+ antiporter and Na+-(HCO–3)n symporter regulate intracellular pH in mouse medullary thick limbs of Henle. Am J Physiol 1990;258:F445–F456.
- Lopes AG, Siebens AW, Giebisch G, Boron WF: Electrogenic Na/HCO3 cotransport across basolateral membrane of isolated perfused Necturus proximal tubule. Am J Physiol 1987;253:F340–F350.
- Kikeri D, Zeidel ML, Ballermann BJ, Brenner BM, Hebert SC: pH regulation and response to AVP in A10 cells differ markedly in the presence vs. absence of CO2-HCO–3. Am J Physiol 1990;259:C471-C483.
Article / Publication Details
First-Page Preview
Published online: July 03, 1998
Issue release date: 1998
Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)
For additional information: https://www.karger.com/CPB
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