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Original Paper

Transplantation in utero of Fetal Human Hematopoietic Stem Cells into Mice Results in Hematopoietic Chimerism

Pixley J.S.a · Tavassoli M.b · Zanjani E.D.a · Shaft D.M.a · Futamachi K.J.a · Sauter T.a · Tavassoli A.a · MacKintosh F.a

Author affiliations

aIoannis A. Lougaris VA Medical Center, Department of Medicine, University of Nevada School of Medicine, Reno, Nev., bVeterans Affairs Medical Center, Department of Medicine, University of Mississippi School of Medicine, Jackson, Miss., USA

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Pathobiology 1994;62:238–244

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: July 09, 1994
Accepted: August 01, 1994
Published online: October 08, 2008
Issue release date: 1994

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: https://www.karger.com/PAT

Abstract

Allogeneic and xenogeneic hematolymphoid chimerism has been achieved in large and small animals using varied techniques to circumvent immune mediated graft rejection by the recipient. We show here the establishment of long-term chimerism in normal mice transplanted in utero with human fetal hematopoietic stem cells (HSC). HSCs from fetal (13-20 weeks’ gestation) human livers were injected into fetal mouse peritoneal cavities on days 11-13 of gestation. Histologic examination demonstrated human chimerism in 29% of 38 live born mice using fluorescein conjugated antibodies to both the CD45 and CD 14 antigens present on human peripheral blood (PB) cells. Further investigation using flow cytometric analysis of cells from 70 mice transplanted in utero revealed 28% of mice greater than 16 weeks of age contained human cells in at least one organ at the following frequencies: 14% PB, 8% bone marrow, 8% spleen and 12% thymus. These data indicate that human fetal HSC can be engrafted into mouse fetuses. Additionally, the identification of circulating human cells 18 months following transplantation supports the engraftment and proliferation of a primitive hematopoietic progenitor.

© 1994 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: July 09, 1994
Accepted: August 01, 1994
Published online: October 08, 2008
Issue release date: 1994

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: https://www.karger.com/PAT


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