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Original Paper

The Remineralization of EDTA–Treated Human Dentine

Kawasaki K.a · Ruben J.b · Stokroos I.c · Takagi O.a · Arends J.b

Author affiliations

aDepartment of Preventive Dentistry, Nagasaki University School of Dentistry, Nagasaki, Japan; bLaboratory for Materia Technica and cLaboratory for Cell Biology and Electron Microscopy, University of Groningen, The Netherlands

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Caries Res 1999;33:275–280

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 14, 1999
Issue release date: July – August

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 1

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: https://www.karger.com/CRE

Abstract

The remineralization of whole human dentine treated with a neutral EDTA solution was investigated: the treatment periods were 15, 30 and 120 min. From the literature it is known that EDTA removes noncollagenous proteins (NCPs) from dentine powders. In order to extract more phosphoproteins from dentine lesions, in part of this work samples were also treated with 1 M NaCl or 4 M guanidine chloride solutions. All the dentine samples after the treatments mentioned were immersed subsequently in a remineralizing solution without fluoride for 2 weeks and microradiographed. To investigate the effects of fluoride, samples treated with EDTA for 120 min were also remineralized with 2ppm fluoride in solution. The results presented show that: (1) Measurable remineralization did not occur when fluoride was free in the remineralizing solution. Because remineralization occurred neither at the lesion front nor in the nonmineral part of the surface–softened tissue, presumably the NCPs (inhibitors) of underlying dentine diffused into the tissue during the remineralization period. (2) 2ppm fluoride caused remineralization at the lesion front. In this case we assume fluoride either acted as nucleating agent or overcame the NCP effects at the lesion front.


References

  1. Chang SR, Holland BH, Clarkson BH: Effects of phosphophoryn on collagen fibril calcium deposition. J Dent Res 1998;77(special issue A): 176.
  2. Clarkson BH, Feagin FF, McCurdy SP, Sheetz JH, Speirs R: Effects of phosphoprotein moieties on the remineralization of human root caries. Caries Res 1991;25:166–173.
    External Resources
  3. Daculsi G, Kerebel B, Le Cabellec MT, Kerebel LM: Qualitative and quantitative data on arrested caries in dentine. Caries Res 1979;13: 190–202.
    External Resources
  4. DeSteno CV, Fragin F, Butler WT: Mineralization of dentin, bone and tendon in vitro. Calcif Tissue Res 1975;17:161–163.
    External Resources
  5. Hunter GK, Nyburg SC, Pritzker KPH: Hydroxyapatite formation in collagen, gelatin, and agarose gels. Coll Relat Res 1986;6:229–238.
    External Resources
  6. Inaba D, Ruben J, Takagi O, Arends J: Effect of sodium hypochlorite treatment on remineralization of human root dentine in vitro. Caries Res 1996;30:218–224.
    External Resources
  7. de Josselin de Jong E, ten Bosch JJ, Noordmans J: Optimized microcomputer guided quantitative microradiography on dental mineral tissue slices. Phys Med Biol 1987;32:887–899.
  8. Klont B, ten Cate JM: Release of organic matrix components from bovine incisor roots during in vitro lesion formation. J Dent Res 1990;69: 869–900.
  9. Klont B, ten Cate JM: Remineralization of bovine incisor root lesions in vitro: The role of the collagenous matrix. Caries Res 1991;25:39–45.
    External Resources
  10. Kuboki Y, Fujisawa R, Aoyama K, Sasaki S: Calcium–specific precipitation of dentin phosphoprotein. A new method of purification and the significance for the mechanism of calcification. J Dent Res 1979;58:1926–1932.
    External Resources
  11. Levine RS, Rowles SL: Further studies on the remineralization of human carious dentine in vitro. Arch Oral Biol 1973;18:1351–1356.
    External Resources
  12. Lussi A, Linde A: Mineral induction in vivo by dentine proteins. Caries Res 1993;27:241–248.
  13. McCurdy SP, Clarkson BH, Feagin FF: Comparison of phosphoprotein isolated from mature and immature human tooth roots. Arch Oral Biol 1992;37:1057–1065.
  14. McCurdy SP, Clarkson BH, Speirs RL, Feagin FF: Phosphoprotein extraction from the dentine/ cementum complex of human tooth roots. Arch Oral Biol 1990;35:347–357.
  15. Øgaard B, Arends J, Schuthof J, Rølla G, Ekstrand J, Oliveby A: Action of fluoride on initiation of early enamel caries in vivo. Caries Res 1986; 20:270–277.
    External Resources
  16. Saito T, Arsenault AL, Yamauchi M, Kuboki Y, Crenshaw MA: Mineral induction by immobilized phosphoproteins. Bone 1997;21:305– 311.
  17. Saito T, Yamauchi M, Crenshaw MA: Apatite induction by insoluble dentin collagen. J Bone Miner Res 1998;13:265–270.
  18. Stetler–Stevenson WG, Veis A: Type I collagen shows a specific binding affinity for bovine dentin phosphophorin. Calcif Tissue Int 1986; 38:135–141.
  19. Tveit AB, Selvig KA: In vivo recalcification of dentin demineralized by citric acid. Scand J Dent Res 1981;89:38–42.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 14, 1999
Issue release date: July – August

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 1

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: https://www.karger.com/CRE


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