Kidney and Blood Pressure Research
Measurement of Defined Biochemical Functions along the Nephron
Intranephron Distribution of Purine-Metabolizing Enzymes in RatsTakahashi T. · Kakuno K. · Yamada H. · Endou H.Department of Pharmacology and Medicine, University of Tokyo, Japan
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Article / Publication Details
Published online: November 07, 2008
Issue release date: 1989
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1420-4096 (Print)
eISSN: 1423-0143 (Online)
For additional information: https://www.karger.com/KBR
Abstract
The activities of purine-metabolizing enzymes, 5’-nucleotidase, adenosine deaminase, and purine nucleoside phosphorylase in microdissected rat nephron segments were measured. The specific activity of 5’-nucleotidase was highest in the proximal tubules and the cortical collecting duct, but low in the glomerulus. In contrast, the highest activity of adenosine deaminase was found in the glomerulus. The distribution pattern of purine nucleoside phosphorylase was similar to that of adenosine deaminase. These results suggest that various nephron segments can form adenosine and that the glomerulus exhibits highest capacities to metabolize this nucleoside.
© 1989 S. Karger AG, Basel
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Article / Publication Details
Published online: November 07, 2008
Issue release date: 1989
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1420-4096 (Print)
eISSN: 1423-0143 (Online)
For additional information: https://www.karger.com/KBR
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