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Astrocyte-Neuron Metabolic Pathways

Glucose and Lactate Metabolism in C6 Glioma Cells: Evidence for the Preferential Utilization of Lactate for Cell Oxidative Metabolism

Bouzier A.-K. · Voisin P. · Goodwin R. · Canioni P. · Merle M.

Author affiliations

Laboratoire de Résonance Magnétique des Systèmes Biologiques, UMR 5536, Centre National de la Recherche Scientifique, Université Victor Segalen, Bordeaux, France

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Dev Neurosci 1998;20:331–338

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Article / Publication Details

First-Page Preview
Abstract of Astrocyte-Neuron Metabolic Pathways

Published online: October 30, 1998
Issue release date: July – October 1998

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Abstract

13C and 1H nuclear magnetic resonance spectroscopy (NMR) was used to investigate the metabolism of L-lactate and D-glucose in C6 glioma cells. The 13C enrichment of cell metabolites was examined after a 4-h incubation in media containing 5.5 mM glucose and 11 mM lactate, each metabolite being alternatively labelled with either [1-13C]D-glucose or [3-13C]L-lactate. The results indicated that exogenous lactate was the major substrate for oxidative metabolism. They were consistent with the concept of the existence of 2 pools of both lactate and pyruvate, of which 1 pool was closely connected with exogenous lactate and oxidative metabolism, and the other pool was closely related to glycolysis and disconnected from oxidative metabolism. The molecular basis of this behaviour could be related to different locations for the lactate dehydrogenase isoenzymes, as suggested by their immunohistochemical labelling.


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Article / Publication Details

First-Page Preview
Abstract of Astrocyte-Neuron Metabolic Pathways

Published online: October 30, 1998
Issue release date: July – October 1998

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE


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