Astrocyte-Neuron Metabolic Pathways
Glucose and Lactate Metabolism in C6 Glioma Cells: Evidence for the Preferential Utilization of Lactate for Cell Oxidative MetabolismBouzier A.-K. · Voisin P. · Goodwin R. · Canioni P. · Merle M.
Laboratoire de Résonance Magnétique des Systèmes Biologiques, UMR 5536, Centre National de la Recherche Scientifique, Université Victor Segalen, Bordeaux, France
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13C and 1H nuclear magnetic resonance spectroscopy (NMR) was used to investigate the metabolism of L-lactate and D-glucose in C6 glioma cells. The 13C enrichment of cell metabolites was examined after a 4-h incubation in media containing 5.5 mM glucose and 11 mM lactate, each metabolite being alternatively labelled with either [1-13C]D-glucose or [3-13C]L-lactate. The results indicated that exogenous lactate was the major substrate for oxidative metabolism. They were consistent with the concept of the existence of 2 pools of both lactate and pyruvate, of which 1 pool was closely connected with exogenous lactate and oxidative metabolism, and the other pool was closely related to glycolysis and disconnected from oxidative metabolism. The molecular basis of this behaviour could be related to different locations for the lactate dehydrogenase isoenzymes, as suggested by their immunohistochemical labelling.
Warburg O: On the origin of cancer cells. Science 1956;123:309–314.
- Argiles JM, Azcon-Bieto J: The metabolic environment of cancer. Mol Cell Biochem 1988;81:3–17.
- Vaupel P: Physiological properties of malignant tumours. NMR Biomed 1992;5:220–225.
Hossmann KA, Mies G, Paschen W, Szabo L, Dolan E, Wechler W: Regional metabolism of experimental brain tumors. Acta Neuropath (Berl) 1986;69:139–147.
- Schwartz JP, Lust WD, Lauderdale VR, Passonneau JV: Glycolytic metabolism in cultured cells of the nervous system. II. Regulation of pyruvate and lactate metabolism in the C6-glioma cell line. Mol Cell Biochem 1975;9:67–72.
- Lust WD, Schwartz JP, Passonneau JV: Glycolytic metabolism in cultured cells of the nervous system. Mol Cell Biochem 1975;8:169–176.
- Dringen R, Peters H, Wiesinger H, Hamprecht B: Lactate transport in cultured glial cells. Dev Neurosci 1995;17:63–69.
- Portais JC, Schuster R, Merle M, Canioni P: Metabolic flux determination in C6 glioma cells using carbon-13 distribution upon [1-13C]glucose incubation. Eur J Biochem 1993;217:457–468.
- Pianet I, Merle M, Labouesse J, Canioni P: Phosphorus-31 nuclear magnetic resonance of C6 glioma cells and rat astrocytes: Evidence for a modification of the longitudinal relaxation time of ATP and Pi during glucose starvation. Eur J Biochem 1991;195:87–95.
- Martin M, Portais JC, Labouesse J, Canioni P, Merle M: [1-13C]glucose metabolism in rat cerebellar granule cells and astrocytes in primary culture. Evaluation of flux parameters by 13C and 1H-NMR spectroscopy. Eur J Biochem 1993;217:617–625.
- Malloy CR, Thompson J, Jeffrey F, Sherry A: Contribution of exogenous substrates to acetyl Coenzyme A: Measurement by 13C NMR under non steady state conditions. Biochemistry 1990;29:6756–6761.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–275.
- Passonneau JV, Crites SK: Regulation of glycogen metabolism in astrocytoma and neuroblastoma cells in culture. J Biol Chem 1976;251:2015–2022.
- Merle M, Martin M, Villégier A, Canioni P: [1-13C]glucose metabolism in brain cells: Isotopomer analysis of glutamine from cerebellar astrocytes and glutamate from granule cells. Dev Neurosci 1996:18:460–468.
- Peuhkurinen KJ, Hiltunen JK, Hassinen IE: Metabolic compartmentation of pyruvate in the isolated perfused rat heart. Biochem J 1983;210:193–198.
Lewandowski ED: Metabolic heterogeneity of carbon substrate utilization in mammalian heart: NMR analysis of metabolic equilibria. Am J Physiol 1996;269:H160–H168.
- Damico LA, White LT, Yu X, Lewandowski ED: Chemical versus isotopic equilibrium and the metabolic fate of glycolytic end products in the heart. J Mol Cell Cardiol 1996;28:989–999.
- Clegg JS, Jackson SA: Glucose metabolism and the channeling of glycolytic intermediates in permeabilized L-929 cells. Arch Biochem Biophys 1990;278:452–460.
- McKenna MC, Sonnewald U, Huang X, Stevenson J, Zielke HR: Exogenous glutamate concentration regulates the metabolic fate of glutamine in astrocytes. J Neurochem 1996;66:386–393.
Tholey G, Roth-Schechter BF, Mandel P: Activity and isoenzyme pattern of lactate dehydrogenase in neurons and astroblasts cultured from brains of chick embryos. J Neurochem 198 1;36:77–81.
Ovadi J, Orosz F: A new concept for control of glycolysis; in Agius L, Sherratt HSA (eds): Channelling in intermediary metabolism. London, Portland Press Research Monograph, 1996, pp 237–268.
- Ross BD, Higgins RJ, Boggan JE, Willis JA, Knittel B, Unger SW: Carbohydrate metabolism of the rat C6 glioma. An in vivo 13C and in vitro 1H magnetic resonance spectroscopy study. NMR Biomed 1988;1:20–26.
- Magistretti PJ, Pellerin L: Cellular bases of brain energy metabolism and their relevance to functional brain imaging: Evidence for a prominent role of astrocytes. Cereb Cortex 1996;6:50–61.
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