Astrocyte-Neuron Metabolic Pathways
Studies of the Source of Glucose in the Extracellular Compartment of the Rat BrainFillenz M. · Lowry J.P.
University Laboratory of Physiology, University of Oxford, Oxford, UK
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Rats were implanted in the striatum with a glucose biosensor glued to a dialysis probe. Changes in extracellular glucose concentration in response to either neuronal stimulation or 3-min periods of hypoxia and hyperoxia were compared when the dialysis probe was perfused with either artificial cerebrospinal fluid (aCSF) alone or aCSF with the addition of the β-adrenoceptor antagonist propranolol. Propranolol had no effect on basal levels of glucose or the changes in glucose produced by hypoxia and hyperoxia, which are attributed to changes in the utilization of glucose. Following neuronal activation there is an initial reduction followed by a delayed, prolonged increase in glucose which is suppressed by propranolol. The results suggest that propranolol has no effect on glucose utilization, but blocks the delivery of glucose from astrocytes.
- Sokoloff L: The brain as a chemical machine. Prog Brain Res 1992;94:19–33.
- Magistretti PJ, Pellerin L: Cellular bases of brain energy metabolism and their relevance to functional brain imaging: Evidence for a prominent role of astrocytes. Cereb Cortex 1996;6:50–61.
- Fox PT, Raichle ME: Focal physiological uncoupling of cerebral blood flow and oxidative metabolism during somatosensory stimulation in human subjects. Proc Natl Acad Sci USA 1986;83:1140–1144.
- Fox PT, Raichle ME, Mintun MA, Dence C: Non-oxidative glucose consumption during focal physiologic neural activity. Science 1988;241:462–464.
- Prichard J, Rothman D, Novotny E, Petroff O, Kuwaraba T, Avison M, Howseman A, Hanstock C, Shulman R: Lactate rise detected by 1H NMR in human visual cortex during physiologic stimulation. Proc Natl Acad Sci USA 1991;88:5829–5831.
- Sappey-Marinier D, Calabrese G, Fein G, Hugg JW, Biggins C, Weiner MW: Effect of photic stimulation on human visual cortex lactate and phosphates using 1H and 31P magnetic resonance spectroscopy. J Cereb Blood Flow Metab 1992;12:584–592.
- Fellows LK, Boutelle MG, Fillenz M: Physiological stimulation increases non-oxidative glucose metabolism in the brain of the freely moving rat. J Neurochem 1993;60:1258–1263.
- Fray AE, Forsyth RJ, Boutelle MG, Fillenz M: The mechanisms controlling physiologically stimulated changes in rat brain glucose and lactate: A microdialysis study. J Physiol 1996;496:49–57.
- Kuhr WG, Korf J: Extracellular lactic acid as an indicator of brain metabolism: Continuous on-line measurement in conscious, freely moving rats with intrastriatal dialysis. J Cereb Blood Flow Metab 1988;8:130–137.
- Dringen R, Gebhardt R, Hamprecht B: Glycogen in astrocytes: Possible function as lactate supply for neighboring cells. Brain Res 1993;623:208–214.
- Forsyth RJ, Bartlett K, Burchell A, Scott HM, Eyre JA: Astrocytic glucose-6-phosphatase and the permeability of brain microsomes to glucose-6-phosphate. Biochem J 1993;294:145–151.
- Forsyth RJ: Astrocytes and the delivery of glucose from plasma to neurons. Neurochem Int 1996;28:231–241.
- Lowry JP, Fillenz M: Evidence for the uncoupling of oxygen and glucose utilization during neuronal activation in rat striatum. J Physiol 1997;498:497–501.
Fillenz M, Lowry JP: The effect of mild hypoxia and hyperoxia on local cerebral blood flow and brain extracellular glucose. J Neurochem 1997;69:S114.
Lowry JP, Demestre M, Fillenz M: The relation between cerebral blood flow and extracellular glucose in rat striatum during mild hypoxia and hyperoxia. Dev Neurosci 1998 (in press).
Lowry JP, O’Neill RD, Boutelle MG, Fillenz M: Continuous monitoring of extracellular glucose concentrations in the striatum of freely moving rats with an implanted glucose biosensor. J Neurochem 1998 (in press).
- Morton DB, Griffiths PHM: Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment. Vet Rec 1985;116:431–436.
- de Boer J, Damsma G, Fibiger HC, Timmerman N, de Vries JB, Westerink BHC: Dopaminergic-cholinergic interactions in the striatum: The critical significance of Ca++ concentration in brain microdialysis. Arch Pharmacol 1990;342:528–534.
- Fray AE, Boutelle MG, Fillenz M: Extracellular glucose turnover in the striatum of unanaesthetised rats measured by quantitative microdialysis. J Physiol 1997;504:721–726.
- McKenna MC, Tildon JT, Stevenson JH, Hopkins IB: Energy metabolism in cortical synaptic terminals from weanling and mature rat brain: Evidence for multiple compartments of tricarboxylic acid cycle activity. Dev Neurosci 1994;16:291–300.
- Schurr A, Payne RS, Miller JJ, Rigor BM: Brain lactate, not glucose, fuels recovery of synaptic function from hypoxia upon reoxygenation: An in vitro study. Brain Res 1997;744:105–111.
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