Developmental Neuroscience

 

Caspase Regulation of Neuronal Progenitor Cell Apoptosis

D’Sa-Eipper C. · Roth K.A.

Author affiliations

Department of Pathology, Division of Neuropathology, Washington University School of Medicine, St. Louis, Mo., USA

Keywords: Caspase-3Caspase-9BaxProgrammed cell deathNeuronal stem cells

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Dev Neurosci 2000;22:116–124
https://doi.org/10.1159/000017433

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: February 15, 2000
Issue release date: February 2000

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Abstract

Programmed cell death (apoptosis) of both proliferating neuroblasts and postmitotic neurons is essential for normal nervous system development. To study the molecular regulation of apoptosis in neuronal progenitor cells, we developed a flow cytometric assay capable of distinguishing between viable, apoptotic, and necrotic cell populations. Incubation of freshly dissociated telencephalic cells from gestational day 12–13 mouse embryos with either cytosine arabinoside (AraC) or staurosporine caused a marked increase in the percentage of apoptotic cells. Both drugs induced caspase-3 activation, as determined by in vitro cleavage of a caspase-3 substrate and immunocytochemical detection of activated caspase-3. Treatment of telencephalic cells with the broad caspase inhibitor BAF, blocked caspase-3 activation and protected cells against both AraC and staurosporine-induced apoptotic death. These results indicate that neuronal progenitors possess a caspase-dependent apoptotic pathway, the activation of which may regulate neuronal progenitor cell numbers in vivo.

© 2000 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: February 15, 2000
Issue release date: February 2000

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 1

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

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2000, Vol.22, No. 1-2

February 2000

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