Annals of Nutrition and Metabolism

Original Paper

Zinc Transfer among Proteins in Rat Duodenum Mucosa

Seal C.J. · Heaton F.W.

Author affiliations

Department of Biological Sciences, University of Lancaster, Lancaster, UK

Related Articles for ""

Ann Nutr Metab 1987;31:55–60

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 12, 1986
Accepted: May 20, 1986
Published online: November 19, 2008
Issue release date: 1987

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: https://www.karger.com/ANM

Abstract

Duodenums from freshly killed stock colony rats were incubated in Krebs buffer containing carrier-free 65ZnCl2 for periods of time between 5 and 60 s. Mucosa cytosol was separated and fractionated by gel filtration on Sephadex G-75.65Zn was present in two main bands of protein with molecular weights of about 45,000 and 6,500, but the ratio between them rose progressively with time of exposure to the isotope. Addition of metabolic inhibitors greatly lowered the 45,000/6,500 ratio for 65Zn. It is concluded that when zinc enters the mucosal cytoplasm during absorption, it rapidly binds to the 6,500 protein in a passive way and is then transferred to the protein of molecular weight 45,000 by an active process.

© 1987 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 12, 1986
Accepted: May 20, 1986
Published online: November 19, 2008
Issue release date: 1987

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: https://www.karger.com/ANM


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