Annals of Nutrition and Metabolism

Original Paper

Effects in Rats of Dietary Protein Inadequacy on Lactose Production, Milk Volume and Components of the Lactose Synthetase Complex (EC 2.4.1.22)

Grimble R.F.a · Mansaray Y.K.C.b

Author affiliations

aDepartment of Nutrition, School of Biochemical and Physiological Sciences, University of Southampton, UK; bDepartment of Chemistry, Njala University College, University of Sierra Leone, West Africa

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Ann Nutr Metab 1987;31:179–184

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 18, 1986
Accepted: September 14, 1986
Published online: November 19, 2008
Issue release date: 1987

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: https://www.karger.com/ANM

Abstract

Lactose synthetase (LS) is a complex of α-lactalbumin and N-acetylglucosamine galactosyl transferase (GT). LS is the rate-limiting enzyme in lactose biosynthesis. Lactose is the main osmotic component of milk and may thereby affect milk volume. Dietary protein inadequacy reduces milk volume and lactose content. The study investigates the role of LS activity in these phenomena. Lactating rats were fed adequate and inadequate amounts of protein of high (milk protein) and low (cereal protein) quality. After 14 days LS and GT activity of mammary tissue was measured. Milk volume and lactose content was determined. While GT activity was unaffected by diet, LS activity was reduced by low dietary protein quality. Addition of bovine α-lactalbumin to tissue incubates largely restored the reduced LS activities. Milk volume was affected in a parallel manner to LS activity. A highly significant positive correlation existed between LS activity and total lactose production (r = 0.794; p < 0.001). Dietary protein inadequacy indirectly reduces milk volume by means of a direct effect on the lactalbumin part of the LS complex and, subsequently, lactose biosynthesis.

© 1987 S. Karger AG, Basel




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 18, 1986
Accepted: September 14, 1986
Published online: November 19, 2008
Issue release date: 1987

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: https://www.karger.com/ANM


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