Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Original Paper

Measurement of Neutrophil Content in Brain and Lung Tissue by a Modified Myeloperoxidase Assay

Kuebler W.M.a · Abels C.a · Schuerer L.a · Goetz A.E.b

Author affiliations

aInstitute for Surgical Resarch, and bInstitute of Anesthesiology, Klinikum Grosshadern, University of Munich, Germany

Related Articles for ""

Int J Microcirc 1996;16:89–97

Do you have an account?

Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.



Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.



To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.


Save over 20% compared to the individual article price.
Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00


Select

Subscribe

For eJournal Archive and eJournal Backfiles information please contact service@karger.com

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 20, 1995
Accepted: December 15, 1995
Published online: November 25, 2008
Issue release date: 1996

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0167-6865 (Print)
eISSN: 1573-1057 (Online)

For additional information: https://www.karger.com/IJC

Abstract

Myeloperoxidase (MPO) activity is assessed for the quantification of neutrophil accumulation in tissues. In particular, it may be used to support in vivo data on leukocyte kinetics obtained by intravital microscopy and to clarify whether phenomena observed on the organ surface reflect the situation of the whole organ microcirculation. Previous measurements of MPO activity were limited by interference with other peroxidases and by inhibition of MPO activity by specific enzymes. To circumvent these limitations, a modified assay was devised that combined a two-step tissue homogenization technique with heat incubation in a continuous photometric measurement. MPO activity was quantified in neutrophils isolated from rat and rabbit whole blood, rat brain and rabbit lung and compared with intravital microscopic data on leukocyte accumulation. The modified assay is characterized by high reproducibility, strong correlation of MPO activity with number of neutrophils and full recovery of neutrophils added to tissue homogenate. MPO activity per neutrophil was 342.9 ± 11.7 mU/106 cells in rats and 40.3 ± 0.8 mU/106 cells in rabbits. MPO activity in tissue was significantly lower in rat brains (18.9 ± 29.7 mU/g) as compared to rabbit lungs (741 ± 67 mU/g). Whereas global cerebral ischemia/reperfusion did not increase MPO activity in rat brain (18.1 ± 26.1 mU/g), intravenous infusion of cobra venom factor (1,447 ± 407 mU/g) or endotoxin (1,439 ± 285 mU/g), enhanced MPO activity in rabbit lung. These results parallel microcirculatory data from the organ surface. Therefore they supplement the intravital microscopic observations by demonstrating that these are indeed representative of deeper parenchymal tissue areas.

© 1996 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 20, 1995
Accepted: December 15, 1995
Published online: November 25, 2008
Issue release date: 1996

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0167-6865 (Print)
eISSN: 1573-1057 (Online)

For additional information: https://www.karger.com/IJC


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.