Hormone Research in Paediatrics
Original Paper
Dopamine Inhibits Thyrotropin-Releasing Hormone Release from Rat Adrenal Gland in vitroMitsuma T. · Sun D.H. · Nogimori T. · Chaya M. · Ohtake K. · Hirooka Y.Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi, Japan
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Article / Publication Details
Received: May 15, 1986
Accepted: September 26, 1986
Published online: November 28, 2008
Issue release date: 1987
Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
Abstract
The effects of dopamine on the release of thyrotropin-releasing hormone (TRH) from the rat adrenal gland were studied in vitro. The rat adrenal glands were incubated in medium 199 with 1.0 mg/ml of bacitracin and 100 µg/ml of ascorbic acid (pH 7.4) (medium) for 20 min. The amount of TRH release into the medium was measured by radioimmunoassay. The immunoreactive TRH (ir-TRH) release from the rat adrenal gland was inhibited significantly in a dose-related manner with the addition of dopamine and enhanced with the addition of pimozide or domperidone to the medium. Dopamine’s effects on ir-TRH release from the adrenal gland were blocked with the addition of pimozide or domperidone. The elution profile of methanol-extracted rat adrenal gland was identical to that of synthetic TRH. The findings suggest that the dopaminergic system inhibits TRH release from the rat adrenal gland.
© 1987 S. Karger AG, Basel
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Article / Publication Details
Received: May 15, 1986
Accepted: September 26, 1986
Published online: November 28, 2008
Issue release date: 1987
Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
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