Hormone Research in Paediatrics
Original Paper
Regulation of Growth Hormone Secretion in Human Trophoblastic Cells in CultureEvain-Brion D.a · Alsat E.a · Mirlesse V.a · Dodeur M.a · Scippo M.L.b · Hennen G.b · Frankenne F.baLaboratoire de Physiopathologie du Développement, Ecole Normale Supérieure, Paris, France; bLaboratoire d’Endocrinologie, Centre Hospitalier Universitaire, Liège, Belgique
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Article / Publication Details
Received: February 14, 1990
Accepted: May 02, 1990
Published online: December 02, 2008
Issue release date: 1990
Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
Abstract
In this study, we have cultured in vitro purified trophoblastic cells from first-trimester and term human placenta. These cells were obtained by specific enzymatic digestion and centrifugation through a Percoll gradient. Using 2 specific monoclonal antibodies, the pituitary 22-kD growth hormone (GH) and the placental GH variant were assayed in the culture medium by radioimmunoassay. After 48 h of culture, only the placental GH variant was measured in the medium corresponding to first-trimester placenta (3.4 ng/24 h/105 cells). Surprisingly, an immunoactivity pattern of pituitary GH type was found in 3 out of 5 media conditioned with term placenta cells, while GH immunoactivity was very low, around the detection level, in the 2 others. These secretions are not modified with the time in culture and the state of differentiation of the cells from cytotrophoblast to syncytiotrophoblast. Neither in early nor in term placenta does the addition of GH-releasing factor (10-6M in the culture medium) stimulate the secretion of pituitary 22-kD GH or placental GH variant.
© 1990 S. Karger AG, Basel
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Article / Publication Details
Received: February 14, 1990
Accepted: May 02, 1990
Published online: December 02, 2008
Issue release date: 1990
Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0
ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)
For additional information: https://www.karger.com/HRP
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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