Nephron
Original Paper
Glomerular Localization of Platelet Factor 4 in Streptococcal NephritisMezzano S.a · Burgos M.E.a · Ardiles L.a · Olavarria F.a · Concha M.a · Caorsi I.a · Aranda E.b · Mezzano D.baDivision of Nephrology and Department of Pathology, School of Medicine, Universidad Austral, Valdivia; bDepartment of Hematology-Oncology, School of Medicine, Universidad Catolica, Santiago, Chile
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Article / Publication Details
Accepted: August 16, 1991
Published online: December 11, 2008
Issue release date: 1992
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1660-8151 (Print)
eISSN: 2235-3186 (Online)
For additional information: https://www.karger.com/NEF
Abstract
Since platelet factor 4 (PF4), a cationic (pI 7.6) platelet secretory protein, binds avidly to glomerular polyanions both in vitro and in vivo, and is implicated in neutrophil chemotaxis, we studied by indirect immunofluorescence microscopy the presence of PF4 deposits in glomeruli of patients with poststreptococcal nephritis (APSGN). Goat antihuman PF4 serum was used as primary antibody and fluorescein-conjugated IgG fraction of rabbit antigoat IgG as second antibody. Controls consisted of nonimmune goat serum or anti-PF4 serum preabsorbed with human PF4, as primary antibodies. Glomerular deposits of PF4 were demonstrated in renal tissues obtained by biopsy in 14 of 20 patients studied; the deposits were particularly intense in 9 patients. PF4 was bound to the mesangium and to the capillary walls. There was a significant positive correlation between intraglomerular deposits of PF4 and the levels of proteinuria (p = 0.024). These findings provide further evidence for a role of platelets in the pathogenesis of APSGN and suggest that PF4 may contribute to alter the glomerular permeability in this disease.
© 1992 S. Karger AG, Basel
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Article / Publication Details
Accepted: August 16, 1991
Published online: December 11, 2008
Issue release date: 1992
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1660-8151 (Print)
eISSN: 2235-3186 (Online)
For additional information: https://www.karger.com/NEF
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