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Original Paper

Anthocyanin-Rich Aronox Extract from Aroniamelanocarpa E Protects against 7β-Hydroxycholesterol-Induced Apoptosis of Endothelial Cells

Zapolska-Downar D.a, b · Nowicka G.a · Sygitowicz G.b · Jarosz M.a

Author affiliations

aNational Institute of Food and Nutrition, and bDepartment of Biochemistry and Clinical Chemistry, Medical University, Warsaw, Poland

Related Articles for ""

Ann Nutr Metab 2008;53:283–294

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 07, 2008
Accepted: October 23, 2008
Published online: January 14, 2009
Issue release date: February 2009

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM

Abstract

Aims: It has been previously reported that dietary polyphenolic compounds have a positive effect in cardiovascular diseases, although the underlying mechanisms remain unknown. In this study, the effects of Aronox, an anthocyanin-rich extract from Aronia melanocarpa E, on human umbilical vein endothelial cell (HUVEC) apoptosis was investigated. Methods: Apoptosis was measured in HUVEC pretreated with Aronox and than treated with 7β-hydroxycholesterol (20 μg/ml). For the evaluation of apoptosis we used the cell binding of annexin V, the propidium iodide uptake method and the TUNEL assay. We also measured the generation of reactive oxygen species (ROS) in the cells, using fluorescent probe 2′,7′-dichlorofluorescein acetate. Moreover, alteration of the mitochondrial membrane potential with subsequent cytochrome c release, activation of caspase-3, and the level of anti-apoptotic Bcl-2 were investigated. Results: Aronox significantly decreased 7β-hydroxycholesterol-induced apoptosis and ROS generation, and ameliorated the collapse of the mitochondrial transmembrane potential with subsequent inhibition of cytochrome c release. Furthermore, down-regulation of Bcl-2 and up-regulation of caspase-3 activation were reversed by Aronox. Conclusion: The protective action of the extract against apoptosis in endothelial cells may contribute to the potential benefits drawn from consumption of plant polyphenols.

© 2009 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 07, 2008
Accepted: October 23, 2008
Published online: January 14, 2009
Issue release date: February 2009

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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