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Bladder Cancer

Urinary Cytokeratin 20 as a Marker for Transitional Cell Carcinoma

Rotem D.a · Cassel A.a · Lindenfeld N.a · Mecz Y.b · Sova Y.c · Resnick M.c · Stein A.b

Author affiliations

aHematology Laboratory and Departments of bUrology and cPathology, Carmel Medical Center, Haifa, Israel

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Eur Urol 2000;37:601–604

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Article / Publication Details

First-Page Preview
Abstract of Bladder Cancer

Published online: April 04, 2000
Issue release date: May 2000

Number of Print Pages: 4
Number of Figures: 1
Number of Tables: 3

ISSN: 0302-2838 (Print)
eISSN: 1873-7560 (Online)

For additional information: https://www.karger.com/EUR

Abstract

Objectives: To determine if detection of cytokeratin 20 (CK20) gene expression, by reverse transcriptase–polymerase chain reaction (RT–PCR) in urine from transitional cell carcinoma (TCC) patients, can provide a new noninvasive tool for the follow–up of patients with urothelial carcinoma of the bladder.

Methods: Urine was collected from 95 patients previously diagnosed as TCC during their follow–up, and from 27 healthy volunteers. All patients had a transurethal resection of tumor or biopsies obtained from ‘suspicious’ areas in the bladder. RNA was extracted from cells collected from the urine and RT–PCR was performed with specific primers for the amplification of cytokeratin 8, a general marker for epithelial cells, and of CK 20, a marker for TCC urothelium.

Results: CK20 expression was detected in 86.7% of TCC patients, and only in 3.3% of healthy volunteers (specificity 96.7%). Strong correlation was found between tumor grade and expression of CK20 in urine. All grade III and IV tumors demonstrated positive CK20 expression (100% sensitivity), whereas the sensitivity for lower grades was between 71 and 80%. Among 11 patients with a previous biopsy–proven diagnosis of TCC and a current negative biopsy, in 9 patients CK20 expression was detected. Further follow–up of these patients for a period of 6 months revealed recurrence of TCC in 4 patients.

Conclusion: CK20 detection in urine cells is a simple, noninvasive method with a high potential to become the marker of choice for monitoring and follow–up of TCC patients. More information is needed regarding CK20 expression in nonmalignant urological disease, to evaluate its use for routine screening purposes.


References

  1. Bonner RB, Hemstreet GP, Fradent Y, Rao JY, Min KW, Hurst RE: Bladder cancer risk assessment with quantitative fluorescence image analysis of tumor markers in exfoliated bladder cells. Cancer 1993;72:2461–2469.
  2. Klein A, Zemer R, Buchumensky V, Klaper R, Nissenkorn I: Expression of cytokeratin 20 in urinary cytology of patients with bladder carcinoma. Cancer 1998;82:349–354.
  3. Miettinen M: Keratin 20: Immunohistochemical marker for gastrointestinal, urothelial and Merkel cell carcinomas. Mod Pathol 1995;8: 384–388.
  4. Moll R, Lowe A, Laufer J, Franke WW: Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992;140:427–447.
  5. Harnden P, Eardley I, Joyce AD, Southgate J: Cytokeratin 20 as an objective marker of urothelial dysplasia. Br J Urol 1996;78:870– 875.
  6. Funaki NO, Tanaka J, Itami A, Kasamatsu T, Ohshio G, Onodera H, Monden K, Okino T, Imamura M: Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription–polymerase chain reaction targeting cytokeratin–20 mRNA. Life Sci 1997;60: 643–652.
  7. Gregoire M, Fradet Y, Meyer F, Tetu B, Bois R, Bedard G, Charrois R, Naud A: Diagnostic accuracy of urinary cytology, and deoxyribonucleic acid flow cytometry and cytology on bladder washing during follow–up for bladder tumors. J Urol 1997;157:1660–1664.
  8. Walther PJ: Editorial: ‘Wildcatting’ for breakthrough in urothelial cancer detection and management – A frustrating business. J Urol 1995;154:1348–1350.
  9. Wheeless LL, Badalament RA, de Vere White RW, Fradent Y, Tribukait B: Consensus review of the clinical utility of DNA cytometry in bladder cancer. Report of the DNA cytometry consensus conference. Cytometry 1993;14: 478–481.
  10. Landman J, Chang Y, Kavaler E, Droller MJ, Liu BC: Sensitivity and specificity of MN–22, telomerase, and BTA in the detection in human bladder cancer. Urology 1998;52:398–402.
  11. Rahat MA, Lahat N, Gazawi H, et al: Telomerase activity in transitional cell carcinoma patients – A preliminary study. Cancer 1999;85: 919–924.
  12. Mao L, Schoenberg MP, Scicchitano M, Erosan YS, Merlo A, Schwab D, Sidransky D: Molecular detection of primary bladder cancer by microsatellite analysis. Science 1996;271: 659–662.
  13. Bertoglio S, Percivale P, Gambini C, Dicasa D, Meszaros P, Sciallero S, Bruzzi P, Badellino F: Cytokeratin immunostaining reveals micrometastasis in negative hematoxylin–eosin lymph nodes of resected stage I–II (pT2–pT3) colorectal cancer. J Chemother 1997;9:119– 120.

Article / Publication Details

First-Page Preview
Abstract of Bladder Cancer

Published online: April 04, 2000
Issue release date: May 2000

Number of Print Pages: 4
Number of Figures: 1
Number of Tables: 3

ISSN: 0302-2838 (Print)
eISSN: 1873-7560 (Online)

For additional information: https://www.karger.com/EUR


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