Acta Haematologica
Original Paper
Molecular Characterization and Nonradioactive Detection of Beta-Thalassemia in MalaysiaFucharoen S.a · Fucharoen G.a · Ata K.a · Aziz S.b · Hashim S.c · Hassan K.c · Fukumaki Y.aaResearch Laboratory for Genetic Information, Kyushu University, Fukuoka, Japan; bBiochemistry Department, Faculty of Medicine, National University of Malaysia; cepartment of Haematology, Institute for Medical Research, Kuala Lumpur, Malaysia
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Article / Publication Details
Received: November 09, 1989
Accepted: February 07, 1990
Published online: February 20, 2009
Issue release date: 1990
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)
For additional information: https://www.karger.com/AHA
Abstract
The spectrum of β-thalassemia mutations in Malaysia has been determined in 45 β-thalassemia chromosomes using dot blot hybridization of the polymerase chain reaction amplified DNA and direct DNA sequencing. Eleven different molecular defects, including those previously detected in Chinese, Asian Indians, and American blacks, and a novel frameshift mutation causing β°-thalassemia were detected. Since this novel mutation, a T deletion in codon 15 creates a new restriction site for EcoRllenzyme; the mutation could be detected by EcoRll digestion of the appropriate amplified fragment. The results of the present study provide additional information on the molecular heterogeneity of β-thalassemia in this population. We also demonstrated the nonradioactive detection method of the β-thalassemia mutation based upon the digoxigenin-labeled oligonucleotide probes.
© 1990 S. Karger AG, Basel
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Article / Publication Details
Received: November 09, 1989
Accepted: February 07, 1990
Published online: February 20, 2009
Issue release date: 1990
Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0
ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)
For additional information: https://www.karger.com/AHA
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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