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Original Paper

Oxidative Challenge and Glucose-6-Phosphate Dehydrogenase Activity of Preterm and Term Neonatal Red Blood Cells

Ko C.H. · Wong R.P.-O. · Ng P.C. · Li K. · Chui K.M. · Yuen P.M.-P. · Fok T.F.

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Department of Paediatrics, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, Hong Kong, SAR, China

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Neonatology 2009;96:96–101

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 25, 2007
Accepted: July 24, 2008
Published online: March 04, 2009
Issue release date: August 2009

Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 1

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: https://www.karger.com/NEO

Abstract

Background: Recent studies suggest that a low antioxidant level in preterm infants may predispose them to increased oxidative stress and results in hyperbilirubinemia, whereas glucose-6-phosphate dehydrogenase (G6PD) activity was found to be higher in preterm infants than in term infants. Objectives: To evaluate (1) the oxidative effect of α-naphthol on preterm and term red blood cells, and (2) the relationship between G6PD activity and the gestational age of these infants. Methods: G6PD activities were determined in preterm and term infants by a standard diagnostic method. Whole blood samples were incubated with α-naphthol for 2 h and their pre- and post-challenged reduced glutathione (GSH) levels were quantified. Results: The mean G6PD activity in preterm infants (n = 113; 13.52 ± 0.19 U/g Hb; gestational age 30.67 ± 0.28 weeks) was significantly higher than that in term infants (n = 100; 12.36 ± 0.16 U/g Hb; gestational age 39.82 ± 0.14 weeks; p < 0.001). A significantly negative correlation was demonstrated between gestational age and G6PD activity (r = –0.34, p < 0.001). GSH levels of preterm and term subjects were similar at baseline, but were significantly decreased upon challenge with α-naphthol (p < 0.001). The percentage reduction in GSH levels was similar in the various gestational age groups. Conclusions: Our data show that G6PD activities had a negative correlation with gestational age of G6PD-normal infants. The similar response of preterm and term erythrocytes to an α-naphthol challenge indicates the manifestation of an active anti-oxidative pathway mediated by cellular GSH.

© 2009 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 25, 2007
Accepted: July 24, 2008
Published online: March 04, 2009
Issue release date: August 2009

Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 1

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: https://www.karger.com/NEO


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