Nephron Experimental Nephrology
Original Paper
Renal and Urinary Glycosaminoglycans in an Experimental Model of Chronic Renal Failure in RatsMichelacci Y.M.a · Cadaval R.A.M.b · Rovigatti R.M.a · Kohlman O.baDisciplina de Biologia Molecular, Departamento de Bioquímica, and bDisciplina de Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
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Exp Nephrol 2001;9:40–48
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Article / Publication Details
First-Page Preview
Published online: October 06, 2000
Issue release date: October 2000
Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 4
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
Abstract
The present paper reports the glomerular and renal individual glycosaminoglycan levels in an experimental model of chronic renal failure (CRF) that was induced in Wistar rats by five-sixths mass ablation. Glycemia, body weight, blood systolic pressure and urinary excretions of creatinine, albumin and glycosaminoglycans were measured for 12 weeks. At the end of the experiment, the weight and the glycosaminoglycan composition of the kidneys were determined. In control rats, heparan sulfate was the main glycosaminoglycan found both in whole kidney and isolated glomeruli, with trace amounts of dermatan sulfate. Isolated glomeruli presented higher heparan sulfate concentrations than whole kidney (expressed as mg/g dry weight). In CRF rats, albuminuria appeared from the 2 week on, and dermatan sulfate and chondroitin sulfate contents of the kidney increased, whereas heparan sulfate levels remained unaltered. Changes in urine glycosaminoglycans (heparan sulfate, chondroitin sulfate and dermatan sulfate) were not statistically significant. The increase in glomerular dermatan sulfate and chondroitin sulfate observed in this experimental model could be related to the mechanisms involved in the glomerulosclerosis and proteinuria that occur in CRF.
© 2000 S. Karger AG, Basel
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Article / Publication Details
First-Page Preview
Published online: October 06, 2000
Issue release date: October 2000
Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 4
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
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