Pathobiology

Original Paper

Free Access

Low-Intensity Pulsed Ultrasound Accelerates Osteoblast Differentiation and Promotes Bone Formation in an Osteoporosis Rat Model

Wu S.a · Kawahara Y.a, b · Manabe T.a · Ogawa K.a · Matsumoto M.a · Sasaki A.a · Yuge L.a, b

Author affiliations

aDivision of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, and bSpace Bio-Laboratories Y.K., Hiroshima University, Hiroshima, Japan

Corresponding Author

Dr. Louis Yuge

Division of Bio-Environmental Adaptation Sciences

Graduate School of Health Sciences, Hiroshima University

1-2-3 Kasumi, Minami-ku, Hiroshima (Japan)

Tel. +81 82 257 5425, Fax +81 82 257 5344, E-Mail ryuge@hiroshima-u.ac.jp

Keywords: Core-binding factor A1Low-intensity pulsed ultrasoundNormal human osteoblastsOsteoporosisOvariectomy

Related Articles for ""
Pathobiology 2009;76:99–107
https://doi.org/10.1159/000209387

Abstract

Objective: We examined the effects of low-intensity pulsed ultrasound (LIPUS) on cell differentiation, bone mineralized nodule formation and core-binding factor A1 (Cbfa1) expression in a normal human osteoblast (NHOst) cell line and bone formation in an osteoporosis animal model. Methods: NHOst cells were cultured in vitro in medium with or without LIPUS stimulation. The ultrasound stimulation frequency was 1.0 MHz at an intensity of 30 mW/cm2 for 20 min. Rats were divided into a sham-operated group (Sham) and an ovariectomized group (OVX). The right femur was treated with LIPUS (Sham-LIPUS and OVX-LIPUS) and the left femur was left untreated (Sham-CON and OVX-CON). Results: LIPUS stimulation accelerated bone nodule formation and enhanced alkaline phosphatase activity. The expression levels of Cbfa1 decreased and calcification occurred earlier and more frequently in the LIPUS than in the CON groups. The wet weight of the femur increased in OVX rats with LIPUS stimulation. Morphological images showed an increase in trabecular spongiosa in the OVX-LIPUS group. Conclusion: LIPUS accelerated osteogenesis. Moreover, since LIPUS prevents bone loss, it may be a promising treatment for osteoporosis.

© 2009 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: August 12, 2008
Accepted: October 22, 2008
Published online: May 19, 2009
Issue release date: May 2009

Number of Print Pages: 9
Number of Figures: 8
Number of Tables: 1

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: https://www.karger.com/PAT

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2009, Vol.76, No. 3

May 2009

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