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Original Paper

Mizoribine Suppresses the Progression of Experimental Peritoneal Fibrosis in a Rat Model

Takahashi S.a · Taniguchi Y.c · Nakashima A.b · Arakawa T.a · Kawai T.a · Doi S.a · Ito T.d · Masaki T.b · Kohno N.a · Yorioka N.b

Author affiliations

Departments of aMolecular and Internal Medicine and bAdvanced Nephrology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, cDivision of Clinical Pharmacotherapeutics, Department of Pharmaceutical Science, Hiroshima International University, Kure, and dSection of Nephrology, Department of Internal Medicine, Shimane University Faculty of Medicine, Shimane, Japan

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Nephron Exp Nephrol 2009;112:e59–e69

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 12, 2008
Accepted: December 01, 2008
Published online: April 23, 2009
Issue release date: May 2009

Number of Print Pages: 1
Number of Figures: 8
Number of Tables: 2


eISSN: 1660-2129 (Online)

For additional information: https://www.karger.com/NEE

Abstract

Background/Aims: Peritoneal fibrosis is a serious complication of peritoneal dialysis (PD). It has been reported that administration of mizoribine, an effective immunosuppressant, ameliorated renal fibrosis in a rat model of unilateral ureteral obstruction. We therefore examined the effects of mizoribine in an experimental model of peritoneal fibrosis. Methods: 24 rats were given a daily intraperitoneal injection of chlorhexidine gluconate and ethanol dissolved in saline. The rats were divided into three groups (n = 8 per group) that received either vehicle or mizoribine at a dose of 2 or 8 mg/kg once a day. 28 days after the start of the treatments the rats were sacrificed and peritoneal tissue samples collected. Macrophage infiltration (ED1), myofibroblast accumulation (α-smooth muscle actin (SMA)) and expression of type III collagen, transforming growth factor (TGF)-β and monocyte chemotactic protein-1 (MCP-1) were examined by immunohistochemistry. Results: Mizoribine significantly suppressed submesothelial zone thickening and reduced macrophage infiltration. Mizoribine also reduced collagen III+ area and decreased the number of α-SMA+, TGF-β+ and MCP-1+ cells. The magnitude of the changes observed was dose-dependent. Conclusion: The administration of mizoribine prevented the progression of peritoneal fibrosis in this rat model. Mizoribine may represent a novel therapy for peritoneal sclerosis in patients undergoing long-term PD.

© 2009 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 12, 2008
Accepted: December 01, 2008
Published online: April 23, 2009
Issue release date: May 2009

Number of Print Pages: 1
Number of Figures: 8
Number of Tables: 2


eISSN: 1660-2129 (Online)

For additional information: https://www.karger.com/NEE


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