Pathophysiology of Haemostasis and Thrombosis
Original Paper
Absence of Specific Binding of Receptor-Mediated Endocytosis, and of Secretion of Alpha-2-Macroglobulin by Cultured Endothelial CellsMarynen P. · Van der Schueren B. · Van Leuven F. · Cassiman J.J. · Van den Berghe H.Division of Human Genetics, University of Leuven, Belgium
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Article / Publication Details
Received: December 22, 1981
Accepted: January 20, 1982
Published online: April 17, 2009
Issue release date: 1982
Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
Abstract
The presence of an α2-macroglobulin (α2M) receptor, the receptor-mediated endocytosis of α2M-protease complexes, and the secretion of α2M by cultured endothelial cells derived from human umbilical vein and from bovine aorta were assessed with 125I-α2M trypsin. Only very low levels of endocytosis of 125I-α2M trypsin were measured, compared to the levels observed in normal human fibroblast cell layers. The absence of α2M receptors on endothelial cells, as measured in binding experiments at 4 °C, points to the nonconcentrative character of this process. The expression of an α2M receptor could not be induced by plating the cells at low densities or by growing them on collagen gels. The secretion of α2M by endothelial cells could not be detected. The production of α2M by human endothelial cells must, therefore, be lower than 3 ng/l06 cells/48 h, the lower limit of the assay.
© 1982 S. Karger AG, Basel
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Article / Publication Details
Received: December 22, 1981
Accepted: January 20, 1982
Published online: April 17, 2009
Issue release date: 1982
Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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