Pathophysiology of Haemostasis and Thrombosis
Original Paper
Spectrophotometric Method for the Assay of Human Blood Coagulation Factor VIIIvan Dieijen G.a · van Dieijen-Visser M.P.b · Franssen J.a · Hemker H.C.aaDepartment of Biochemistry, University of Limburg, Maastricht; bDepartment of Clinical Chemistry, De Wever Hospital, Heerlen, The Netherlands
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Article / Publication Details
Received: May 03, 1985
Accepted: August 05, 1985
Published online: April 23, 2009
Issue release date: 1987
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
Abstract
A spectrophotometric method for the assay of human blood coagulation factor VIII in plasma is presented. The chromogenic assay for factor VIIL·C in plasma is performed in 3 steps: (1) activation of factor VIII by thrombin; (2) activation of factor X in a mixture of factor X, factor IXa, phospholipids/Ca2+ and plasma containing activated factor VIII, and (3) determination of the rate of factor Xa formation with the chromogenic substrate S2337. Within-assay variation was between 5 and 6.9% for factor VIIL·C activities between 20 and 150%. Clotting and chromogenic factor VIIL·C activities were compared in plasma of 50 normal healthy donors (coefficient of correlation r = 0.83).
© 1987 S. Karger AG, Basel
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Article / Publication Details
Received: May 03, 1985
Accepted: August 05, 1985
Published online: April 23, 2009
Issue release date: 1987
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
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