Medical Principles and Practice

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Antinociceptive and Anti-Inflammatory Effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae)in Experimental Animal Models

Sulaiman M.R.a · Zakaria Z.A.b · Chiong H.S.a · Lai S.K.a · Israf D.A.a · Azam Shah T.M.a

Author affiliations

aDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, and bFaculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Malaysia

Corresponding Author

Dr. Mohd Roslan Sulaiman

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences

Universiti Putra Malaysia

43400 UPM Serdang, Selangor (Malaysia)

Tel. +60 3 8946 8462, Fax +60 3 8943 6178, E-Mail shaza8174@yahoo.com

Keywords: Stachytarpheta jamaicensisAntinociceptive activityAnti-inflammatory activityOpioid mechanism

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Med Princ Pract 2009;18:272–279
https://doi.org/10.1159/000215723

Abstract

Objective: The present study was carried out to explore the antinociceptive as well as the anti-inflammatory effects of an ethanol extract of Stachytarpheta jamaicensis (L.) Vahl (EESJ) using 3 models of nociception and 2 models of inflammation in experimental animals. Materials and Methods: EESJ was prepared by overnight soaking of the oven-dried (50°C; 72 h) ground leaves (500 g) in 80% ethanol (1:5; w/v). The filtrate was evaporated to dryness (50°C), resuspended in distilled water at concentrations to provide the desired doses of 50, 100 and 150 mg/kg. For antinociceptive effects, 3 models were used: acetic acid-induced abdominal writhing, hot-plate- and formalin-induced paw-licking tests; for anti-inflammatory effects, 2 models were used – carrageenan-induced paw edema and cotton-pellet-induced granuloma tests. Appropriate doses were administered intraperitoneally (i.p.) to mice/rats prior to each test. The mechanisms of antinociceptive action of the extract were also investigated by pretreatment with naloxone (5 mg/kg, i.p.). Results: The extract exhibited significant (p < 0.05) antinociceptive activity in all nociceptive models tested with dose-dependent activity observed using the abdominal writhing and formalin tests. Pretreatment with naloxone partially, but significantly (p < 0.05) reversed the antinociceptive activity of the extract when assessed using the abdominal-writhing- and formalin-induced paw-licking tests, and completely inhibited its activity when the hot-plate test was used. The extract also showed significant (p < 0.05) anti-inflammatory activity in both the acute (carrageenan-induced paw edema test) and the chronic (cotton-pellet granuloma test) tests. Conclusion: This study showed the potential of EESJ to exert antinociceptive and anti-inflammatory activities, the former being modulated via peripheral and central mechanisms and involving, in part, activation of the opioid receptor system.

© 2009 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 02, 2007
Accepted: November 25, 2007
Published online: June 02, 2009
Issue release date: June 2009

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 5

ISSN: 1011-7571 (Print)
eISSN: 1423-0151 (Online)

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2009, Vol.18, No. 4

June 2009

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