Pathophysiology of Haemostasis and Thrombosis
Original Paper
Severe von Willebrand’s Disease: SDS Agarose Multimer AnalysisRedaelli R. · Pezzetti L. · Caimi T. · Baudo F. · de Cataldo F.Department of Haematology Talamona, Ospedale Ca’ Granda Niguarda, Milan, Italy
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Article / Publication Details
Received: December 06, 1986
Accepted: March 04, 1987
Published online: April 23, 2009
Issue release date: 1987
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
Abstract
In severe von Willebrand’s disease (vWD), the multimeric analysis of von Willebrand factor (vWF) has been hindered by the low content of the protein although different patterns have been observed by radiocrossed immunoelectrophoresis (RCIE). Cryoprecipitate and vWF concentrate were obtained from the plasma of a severe vWD patient, that on RCIE had only the more anodic forms. The sodium dodecylsulfate (SDS) agarose analysis evidences the presence of the main bands, a relative increase of the smaller forms and a lesser definition of some minor bands. These findings suggest the possibility of structural abnormalities in severe vWD. The study of cryoprecipitates could be a means to evidence the presence of multimers and the extent of the eventual pattern variability.
© 1987 S. Karger AG, Basel
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Article / Publication Details
Received: December 06, 1986
Accepted: March 04, 1987
Published online: April 23, 2009
Issue release date: 1987
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)
For additional information: https://www.karger.com/PHT
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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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