Engineering Bacterial Signals and SensorsSalis H.a · Tamsir A.b · Voigt C.aaDepartment of Pharmaceutical Chemistry and bBiochemistry, Cell & Developmental Biology, and Genetics (Tetrad) Graduate Program, University of California, San Francisco, Calif., USA
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Article / Publication Details
Published online: June 02, 2009
Cover Date: 2009
Number of Print Pages: 32
Number of Figures: 0
Number of Tables: 0
ISBN: 978-3-8055-9132-4 (Print)
eISBN: 978-3-8055-9133-1 (Online)
Abstract
In the emerging field of synthetic biology, a central goal is to reliably engineer bacteria to respond to environmental signals according to a pre-determined genetic program. The sensor systems and genetic circuitry inside bacteria are the ‘eyes’ and ‘brain’ of a new class of biotechnological applications in which bacteria are used as living, self-replicating computers that can beneficially interact with the physical world. These engineered gene networks are constructed by extracting natural sensor systems and other genetic parts from multiple organisms and recombining them into novel configurations. This chapter is a how-to guide. It describes several strategies for engineering new bacterial sensor systems and synthetic gene networks that are capable of sensing a desired stimulus and generating interesting dynamical or pattern-forming responses. We also provide specification sheets describing many two-component and quorum-sensing systems, focusing on the information that one needs to know in order to use them for engineering applications.
© 2009 S. Karger AG, Basel
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Article / Publication Details
Published online: June 02, 2009
Cover Date: 2009
Number of Print Pages: 32
Number of Figures: 0
Number of Tables: 0
ISBN: 978-3-8055-9132-4 (Print)
eISBN: 978-3-8055-9133-1 (Online)
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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