Chemotherapy
Microbiology
Streptomycin Resistance in Some Wild-Type Strains of EnterobacteriaceaeRassekh M. · Pitton J.S.Institute of Medical Microbiology of the University, Geneva
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Article / Publication Details
Published online: May 19, 2009
Issue release date: 1971
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
Abstract
Streptomycin-resistant strains of Enterobacteriaceae bearing R-factors inactivate the antibiotic by an enzymatic mechanism. Streptomycin is inactivated by all the 54 wild-type strains of resistant E. coli we studied, and the inactivation occurs also with the strains (4) which could not transfer their resistance. With Salmonellae, 60% of the wild-type strains studied (20 strains) inactivate the drug. These results are strongly in favour of the hypothesis that streptomycin resistance among wild-type strains of E. coli is probably always due to R-factors, transmissible or not. This is also the case for the majority of wild-type strains of Salmonellae. The enzyme responsible for the inactivation of the drug is localized in the periplasmic space of the resistant cell. The crude enzyme can be released either by osmotic shock or can be obtained in an extract of alumina-ground resistant cells. The only requirements for the inactivation of streptomycin are ATP and Mg++, the reaction taking place efficiently at pH 7.9.
© 1971 S. Karger AG, Basel
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Article / Publication Details
Published online: May 19, 2009
Issue release date: 1971
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
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