Intervirology

Original Paper

Free Access

Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses

Liu S. · Zhang X. · Wang Y. · Li C. · Han Z. · Shao Y. · Li H. · Kong X.

Author affiliations

Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, PR China

Corresponding Author

Shengwang Liu, Division of Avian Infectious Diseases

National Key Laboratory of Veterinary Biotechnology

Harbin Veterinary Research Institute

Chinese Academy of Agricultural Sciences, Harbin 150001 (PR China)

Tel. +86 451 8593 5065, Fax +86 451 8273 4181, E-Mail swliu@hvri.ac.cn

Keywords: Avian infectious bronchitis coronavirusMutationInsertion and deletionRecombinationPathogenicity

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Intervirology 2009;52:223–234
https://doi.org/10.1159/000227134

Abstract

Objective: To monitor and study the molecular epidemiology, evolution and pathogenicity of infectious bronchitis viruses (IBVs) in China in recent years and further our knowledge of the evolution of IBVs. Methods: Thirty-seven IBV isolates were isolated from commercial chickens in China. The isolates were characterized by RT-PCR, sequencing, typing and analyzing the entire S1 gene. In addition, 4 selected IBV isolates were used to experimentally infect the specific pathogen-free chickens to study their pathogenicity. Results: Three types of IBV have been cocirculating in chicken flocks in China in recent years. Unique insertions and deletions in S1 protein regions were identified among different types of IBV. Moreover, a new IBV strain was isolated and identified in a layer hen. S1 gene analysis showed that a recombination event had occurred in the virus’s evolutionary process. In addition, experimental infection has shown that IBV isolates have been nephropathogenic in China in recent years. Conclusion: Mutations, insertions, deletions and recombination of the S1 protein gene contribute to the genetic diversity of IBV in China. Cocirculation of multiple types of IBV in field conditions in China renders its epidemiology and evolution very complicated, indicating the necessity for development of new vaccines or vaccine strategies.

© 2009 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 06, 2008
Accepted: April 03, 2009
Published online: July 03, 2009
Issue release date: July 2009

Number of Print Pages: 12
Number of Figures: 3
Number of Tables: 6

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

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